The reaction between zinc and alizarin red S has been studied spectrophotometrically in an attempt to establish the optimum conditions for determining micro amounts of zinc. Alizarin red S forms a Violet colored chelate with zinc at pH 6.4 ± 0.1 with a λ max of 540 nm. Beer's law is obeyed over a concentration range of 1.8 to 9.2 ppm of zinc. The composition of the chelate is found to be 1:2 and the value of log K is 7.89 ± 0.2 at 25°C, the molar extinction coefficient being 5950 at 540 nm. According to Sandell's notation the sensitivity is 0.010986 μg of zinc per cm. The effect of various diverse ions was also studied.