Studies of the Triplex Formation of Dideoxyadenosine Methyl Phosphonate Stereoisomers with Polyuridylic Acid by ^(31)p NMR Spectroscopy

以磷-31核磁共振訊號為探針來探測由去氧腺嘌呤-3',5'-去氧腺嘌呤甲基磷酸酯與聚尿嘧啶形成之核酸三螺旋

甘魯生(Lou-Sing Kan) ； Paul S. Miller

《中央研究院化學研究所集刊》 41期 (1994/03) Pp. 83-92

https://doi.org/10.6522/BICAS.1994.41.11

摘要

核酸三螺旋的形成闡明了基因治療法的可行性。在生物與醫藥應用上有相當的潛力。本文是要探討以核酸中磷-31原子的核酸共振訊號來探測核酸三螺旋的形成的可行。磷-31的自旋量子數是1/2，在自然界的含量是百分之百。它的敏感度是氫的6.6%，所以很容易以備有傅立葉轉換的核磁共振儀測得。由於所呈現的光譜簡單，可作探針之用。本文即以去氧腺嘌呤-3',5'-去氧腺嘌呤甲基磷酸醋與聚尿嘧啶三螺旋的形成來證實以磷-31核磁共振訊號是一個很好的探針，它可以應用在其他三螺旋的形成上。

並列摘要

Both polyuridylic acid (poly U) and stereoisomers of deoxyadenylyl-3',5' deoxyadenosine methyl phosphonate (R and S) have distinctive ^(31)P NMR signals. Thus, it can be used as indicators for monitoring the triplex formation as a function of temperature. We found that triplex R + 2poly U is more stable than S + 2poly U by observing the changes of both chemical shifts and linewidths at half height.