Quantitative Analysis and Comparison of the Plasma Amino Acids in the Umbilical Cord and Peripheral Veins of the Newborns

新生兒末梢血及臍帶血各種血漿胺基酸之測定值及其比較值

王作仁(Tso-Ren Wang)

《Acta Paediatrica Sinica》 27卷4期 (1986/08) Pp. 294-300

摘要

研究新生兒臍帶血及新生兒末梢血液中之各種血漿胺基酸值卽可做為其營養狀況之極佳參考資料，又可做為進行高營養療法之極佳指標，更重要的是可做為各種先天性胺基酸代謝異常症診斷及治療之用途。我們對於出生於國立臺灣大學醫學院附設醫院之新生兒，取其足月、順產者共200例進行研究。順產後立卽採取臍靜脈血0.5ml，嬰兒出生後卽採取由母親自行決定餵食的內容的方式，共有80%的新生見採混合餵食，其餘全餵食母奶及全餵食嬰兒奶粉者各佔10%。出生後二至四天卽採取末梢靜脈血0.5ml，採血時間是在早上9時至12時之間，採血後卽刻分離血漿，加上四分之一血漿量的10% sulfosalicylic acid，在30分鐘內完成去蛋白的手續，隨卽進行胺基酸的分析，所採用的是Biotronik LC 5000胺基酸分析儀。我們分別定量臍靜脈血中及新生兒末梢血中各種血漿胺基酸的正常值，以平均值標準偏差表之，並列出其範圍，同時算出兩者個別胺基酸之比較值。我們發現出生後2至4天內，除了胱氨酸、絲氨酸及羥輔氨酸外，所有的胺基酸值均普遍下降，其中尤以精氨酸、纈氨酸、異白氨酸、白氨酸、組氨酸、離氨酸、色氨酸及蘇氨酸下降得最為明顯。

並列摘要

Plasma amino acid concentrations of the umbilical cord vein and of peripheral veins of newborn infants can be used to evaluate nutritional status, as guidelines for hyperalimentation and for diagnosis and treatment of the inborn errors of amino acid metabolism. Two hundred healthy term and normal, spontaneously-delivered neonatals born at the National Taiwan University Hospital were studied. Immediately after the spontaneous delivery, a half milliliter of blood was drawn from the umbilical vein. The infants were fed ad libitum after birth. Approximately eighty per cent of them were mixed fed. Half milliliter of blood was drawn from a peripheral vein without fasting. The blood was centrifuged immediately, and the plasma de-proteinized within thirty minutes. Amino acid analyses were performed by an ion exchange amino acid and analyzer. The values of plasma amino acids from both umbilical venous and peripheral venous bloods taken between two and four days of age were analyzed, calculated, and compared. The comparison showed an essential general decline, two to four days after birth, of all amino acids with the exception of cystine, serine and hydroxyproline. Arginine, valine, isoleucine, leucine, histidine, lysine, tryptophane, and threonine showed the most marked decline.

並列關鍵字

plasma amino acids ； umbilical cord veins ； peripheral blood ； quantitative determination ； comparison