A Comparison Among Quantitative, Dye Reduction and Fluorescent Spot Tests for G-6-PD Deficiency

篩檢葡萄糖六磷酸鹽脫氫酶缺乏之比較

曹龍彥(Lon-Yen Tsao) ； 林明燦(Ming-Tsan Lin)

《Acta Paediatrica Sinica》 29卷6期 (1988/12) Pp. 371-375

摘要

在台灣地區，葡萄糖六磷酸鹽脫氫(G-6-PD)缺乏症，咸認是新生兒黃疸，藥物引起之溶血性貧血，及蠶豆中毒症的重要原因。本研究將210人依G-6-PD之活性分成三組：(1)重度酵素缺乏者（酵素缺乏者（酵素活性在正常值之10%以下）。(2)輕度酵素缺乏者（酵素活性在正常值之10~60%之間）。(3)正常者（酵素活性在正常值之60%以上），然後將此每人的血同時用quantitative, dye reduction，及fluorescent spottests檢驗之。其結果顯示：fluorescent spot test在測定男性G-6-PD缺乏者其sensitiviey（敏感性及specificity（特異性）爲100%，而在測定正常女性者其凖確定亦爲100%。此檢驗法又可篩檢出一些輕度酵素缺乏者。作者認爲fluorescent spot test實爲一簡便、經濟且正確之G-6-PD缺乏症的篩檢法。

並列摘要

Glucose-6-phosphate dehydrogenase deficiency continues to be a main cause of neonatal hyperbilitrubinemia, drug-induced hemolytic anemia and favism in Taiwan. In an effort to find a cost efficient and effective G-6-PD deficiency screening means, 210 cases were arbitrarily divided into three classes according to the quantitative activities in red cells, i.e. gross enzyme deficiency (＜10% of normal), partial enzyme deficiency (10-60% of normal) and normal enzyme activities (＞60% of normal). Then each case was screened with quantitative, dye reduction and fluorescent spot tests at the same time.
The results revealed that the fluorescent spot test has 100% sensitivity and specificity in hemizygous males and is 100% effective in ruling out this disorder in normalfemales; it can pick up some (5/30) of the partial enzyme deficiency according to our criteria of fluorescent intensity. Therefore, the fluorescent spot test can be used as a simple, low cost, acceptable, and reliable mass screening means for G-6-FD deficiency.

並列關鍵字

glucose-6-phosphate dehydrogenase (G-6-PD) ； quantitative test ； dye reduction test ； fluorescent spot test ； mass screening