The Isolation and Characterization of Chaperonin 60 from Sweet Potato Roots-Involvement of the Chaperonins in Starch Biosynthesis

甘藷塊根Chaperonin 60之分離及檢定－其澱粉生合成的角色

張世宗(Shih-Chung Chang) ； 林珮君(Pei-Chun Lin) ； 陳翰民(Han-Min Chen) ； 吳建興(Jiann-Shing Wu) ； 莊榮輝(Rong-Huay Juang)

《Botanical Bulletin of Academia Sinica》 41卷2期 (2000/04) Pp. 105-111

https://doi.org/10.7016/BBAS.200004.0105

摘要

由甘藷塊根中可分離得molecular chaperonins cpn60α及cpn60β，并檢定為同質多元體，但兩者的免疫及生物化學性質有相當大的差異性。這兩種cpn在塊根充實的過程中，與澱粉磷解酶同時出現，并以組織定位方法得知位於澱粉粒上。當甘藷開始生合澱粉時，在塊根異常形成層(anomalous cambiun)附近的細胞中，三種蛋白質的量都有顯著增加。以上觀察顯示，chaperonins可能對甘藷澱粉的生合成有重要的生理功能。

關鍵字

造粉體 ； 異常形成層chaperonins ； 組織定位法 ； Ipomoea batatas ； 澱粉生合成 ； 澱粉磷解酶

並列摘要

Molecular chaperonins cpn60α and cpn60β were isolated from sweet potato roots and were characterized as homologous polymers. However, their immunochemical and biochemical properties were divergent. The two chaperonins as well as starch phosphorylase appeared concurrently during the starch-accumulating period and were localized in the starch granule by histochemical methods. Notably, these proteins increased substantially in cells around the anomalous cambium when sweet potato initiated starch biosynthesis. Observations in this study indicated that the chaperonins might be involved in the biosynthesis of starch in sweet potato roots.

並列關鍵字

Amyloplast ； Anomalous cambium ； Chaperonins ； Histochemical method ； Ipomoea batatas ； Starch biosynthesis ； Starch phosphorylase.