- 期刊
A Tissue Culture Protocol for Propagation of a Rare Plant, Lilium speciosum Thunb. var. gloriosoides Baker
組織培養法繁殖臺灣稀有植物－艷紅鹿子百合
並列摘要
Floret explant of a local accession of Lilium speciosum Thunb. var. gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/I 2,4-dichlorophe- noxyacetic acid and 0.25mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1mg/l naphthalene acetic acid, 1 g/l active charcoal and 170 mg/l NaH2PO4. The rare lily was proliferated in vitro by a scale-bulblet cycling propagation method with a multiplication rate of eight times at three-month intervals. Finally, 6000 plantlets have been produced within nine months. We established 200 plants in the greenhouse under misty conditions for a four-week period with a 98% survival rate. These plants grew well and elongated with normal flowers in the second year.
延伸閱讀
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