Characterization of a 10cM Region of Rice Chromosome 5

水稻是世界上最重要的糧食作物之一。又由於它具有較小的基因組（430百萬鹼基對），具備很密的物理與圖及遺傳與圖，以及轉殖系統已被建立，水稻成爲最適合於進行基因級定序分析的作物，台灣參加了世界合作圑隊、進行解讀水稻的遺傳密碼。水稻有十二條染色體，我們所參與的是第五條染色體的大量主序列分析。在第五條染色體短臂約10cM處有一個PAC殖系－P0699E04,本文敘述此殖系被定序與定性的情形。我們以亂槍法製備了2kb與5kb的基因庫，並進行了約4000個定序反應，其中的四分之三的序列被用以進行序列組合，得到了P0699E04的全長爲175,439鹼基對。解讀的結果指出它有28個可能的基因，有些與已知的蛋白質或EST序列類似，而有些則爲全新的蛋白質。另外、我們也研究了包括一般重複序列、反轉錄跳蹈子等重複DNA序列。

關鍵字

序列解讀；大量基因組定序；重複序列；水稻基因組

並列摘要

Rice is a model species for the cereals and a good candidate for genome sequencing due to its relatively small genome (430 Mb), dense physical and genetic maps, and good transgenic systems. As part of an international effort to decode the rice genome, a PAC clone localized at 10cM of chromosome 5 is completely determined for its sequence using shotgun libraries of its two inserts, 2-kb and 5-kb in length. In total 2,998 sequencing reads were used for the assembly of the final sequence, covering 175,439bp. This sequence may code for at least 28 putative proteins, as deduced from computational search for homology with other known coding sequences and EST, or predicted using GenScan package. Also present in this sequence are simple repeats, palindrome and retrotransposons. On the basis of these findings, the gene density in the gene-rich region of rice genome is about 6 kb/gene.

並列關鍵字

Annotation ； High-throughput genome sequencing ； Repetitive sequences ； Retrotransposons ； Rice genome

國際替代計量

Characterization of a 10cM Region of Rice Chromosome 5

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