RAPD Variation in Relation to Population Differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis

紅檜與台灣扁柏RAPD族群分化

黃士穎(shih-Ying Hwang) ； 林惠文(Huei-Wen Lin) ； 郭怡秀(Yi-Shou Kuo) ； 林讚標(Tsan-Piao Lin)

《Botanical Bulletin of Academia Sinica》 42卷3期 (2001/07) Pp. 173-179

https://doi.org/10.7016/BBAS.200107.0173

摘要

本文探討紅檜與台灣扁柏之族群分化問題。紅檜與台灣扁柏的樓蘭山與阿裏山兩個族群以逢機擴增多型性DNA分子標記研究族群分化之問題。RAPD變異以Shannon′s phenotypic index來估算Ho。無論是紅檜或台灣扁柏之樓蘭山族群都較阿裏山族有較高之遺傳變異。上述結果可能與樓蘭山爲紅檜與台灣扁柏之大族群有蘭。基於RAPD之分析發現紅檜樓蘭山與阿裏山族群間有15.14%之分化值，而台灣扁柏樓蘭山與阿裏山族群間分化值爲14.73%。RAPD基因座之高的遺傳變異與族群分化程度可能代表紅檜與台灣扁柏之動態演化有關。

關鍵字

紅檜 ； 台灣扁柏 ； 族群分化 ； 逢機擴增多型性DNA

並列摘要

The population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis based on random amplified polymorphic DNA (RAPD) variation is described. Two populations (Chilanshan and Alishan) of these two Chamaecyparis species are investigated for population differentiation. Shannon's phenotypic index was used to estimate Ho of these two Chamaecyparis species based on RAPD variation. RAPD analysis showed that the Chilanshan population had higher genetic diversity than the Alishan population in both Chamaecyparis species. These results correlated with the large population found in Chilanshan for both species. Based on RAPD analysis, there was 15.13% population differentiation between Chilanshan and Alishan of C. forinosensis compared with 14.73% for C. taiwanensis. Higher levels of genetic variation and population differentiation indicated dynamic evolution in these two Chamaecyparis species in Taiwan as revealed by variation at RAPD loci.

並列關鍵字

Chamaecyparis formosensis ； Chamaecyparis taiwanensis ； Population differentiation ； RAPD