Ultrastructural Study and Lipid Formation of Isochrysis sp. CCMP1324

利用光學及穿透式電子顯微鏡技術，觀察海洋微藻Isochrysis sp.CCMP1324其形態與脂肪形成之過程，發現其脂肪含有多元不飽和脂肪酸，尤其是廿二碳六烯酸(DHA)，爲時下流行之健康食品，對於人體健康維護及水産養殖餌料，提供相當大之助益。在10%CO2之通氣及10klux之連續照明下，細胞中廿二碳六烯酸之含量繼續增加至八天之培養，最終含量爲每升含16毫克。實驗中並探討基脂肪在生長過程之形成與變化，爲首次利用電顯探討等鞭金藻油脂形成之研究報告。

關鍵字

廿二碳六烯酸；等鞭金藻；油脂形成；多元不飽和脂肪酸；超微結構

並列摘要

This study investigates methods for extracting lipids from microalgae and analyzes the effects of culture media as well as culture conditions on PUFA yields and total fatty acid contents. Experimental results of an optimal culturing of Isochrysis spp. were based on a 3.2% salinity culture medium. These microalgae were cultured in a 1-2 L Roux's flat-flask and a 5L jar fermentor. The optimum culture temperature and initial pH for DHA production were 25℃ and 8.0, respectively. Pigments included chlorophylls a and c. The DHA yield increased with cultivation time until the eighth day. Optimum DHA amounts in the cells were reached under aeration with 10% CO2 and with continuous illumination of 10 klux. The biomass dry weight reached 4g per liter of culture, and the DHA production reached 16 mg per liter of culture. Lipid bodies in Isochrysis spp. and related genera were observed during culture by light and transmission electron microscopy; 0.5~3.0 pm sized lipid bodies were confirmed by staining with Sudan Black B in cells from log stage to stationary stage cultures. These results demonstrated that DHAcontaining lipid bodies in cells can be produced and accumulated in marine Isochrysis spp.

並列關鍵字

Docosahexaenoic acid ； Isochrysis sp. ； Lipid formation ； Polyunsaturated fatty acids (PUFA) ； Ultrastructure

國際替代計量

Ultrastructural Study and Lipid Formation of Isochrysis sp. CCMP1324

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