Some Characteristics of Microalgae Isolated in Taiwan for Biofixation of Carbon Dioxide

以台灣分離微藻行二氧化碳生物固定之一些特性

張義宏(Ed-Haun Chang) ； 楊盛行(Shang-Shyng Yang)

《Botanical Bulletin of Academia Sinica》 44卷1期 (2003/01) Pp. 43-52

https://doi.org/10.7016/BBAS.200301.0043

摘要

由台灣之湖泊、池塘、底泥、豬糞廢水、水田、溫泉和海水中分離出二百餘株微藻可以固定二氧化碳。其中由豬糞尿廢水中分離單細胞微藻Chlorella sp.NTU-H15和Chlorella sp.NTU-H25可以在40%CO2中生長。在20% CO2下，生長速率介于0.12至0.22g dry wt1^(-1)d(-1)。此二分离株在5~40% CO2和190-589μmol m^(-2)s(-1)下有相似之光反應。Chlorella sp.NTU-H15生長速率高于Chlorella sp.NTU-H15。并且Chlorella sp.NTU-H15可以耐受高濃度CO2，高細胞濃度、寬廣pH和培養溫度，每升可得1.8克乾重菌體，在15%　CO2下，最大生長速率0.28g dry wt1^(-1)d^(-1)和此生長速率0.27d^(-1)。每mg葉綠素在30℃，700μmol m^(-2)s^(-1)和mM NaHCO3可以產生1.6mMO2。至于每升Chlorella sp.NTU-H25可以產生1.7g乾重菌體，最大生長速率0.25g dry wt1^(-1)d(-1)和此生長速率0.27d^(-1)。可見此兩株微藻適合于高菌體濃度下固定CO2生產菌體。

關鍵字

生物固定 ； 生長速率 ； 微藻 ； Chlorella

並列摘要

To contribute the biological mean of CO2 fixation, more than 200 microalgal isolates were screened from lakes, ponds, sediments, hog wastewater, paddy fields, hot springs, and seawater in Taiwan. Two unicellular microalgae, Chlorella sp. NTU-H15 and Chlorella sp. NTU-H25, were isolated from hog wastewater. In the laboratory, they were able to grow up even in aeration containing CO2 up to 40% and have growth rates of 0.21 to 0.22g dry wt l^(-1)d^(-1) at 2O% CO2. Both algae had the same growth rate in the range from 5 to 4O% CO2 and had a similar light response between 190 and 589 μmol m^(-2) s^(-1). Chlorella sp. NTU-H15 had a higher growth rate than Chlorella sp. NTU-H25 at pH 4.0 and (≥)35℃.Chlorella sp. NTU-H15 was able to tolerate high concentrations of CO2, high cell density, and a broad-range of temperature and pH Each liter of Chlorella sp. NTU-H15 produced 1.8g of dry cell. The maximum growth rate was 0.28g dry wt l^(-1)d^(-1), and the specific growth rate was 0.27 d^(-1)at l5% CO2. Each mg of chlorophyll produced 1.6mM O2 min^(-1) at 700μmol m^(-2)s^(-1) at 30℃ and 10 mM NaHCO3. While each liter of Chlorella sp. NTU-H25 produced 1.7g of dry cell, the maximum growth rate was 0.27g dry wt 1^(-1)d^(-1), and the specific growth rate was 0.27 d^(-1). Both isolates are suitable for dense cultivation to fix CO2 directly and to produce cell biomass.

並列關鍵字

Biofixation ； Carbon dioxide ； Chlorella ； Growth rate and microalga