- 期刊
The Mucilage of Yam (Dioscorea Batatas Decne) Tuber Exhibited Angiotensin Converting Enzyme Inhibitory Activities
山藥黏質多醣具有血管收縮素轉化酶抑制活性
摘要
山藥（Dioscorea batatas Decne）黏質多醣經由抽取與純化步驟，以SDS電泳膠片顯示達到均質，以toluidine blue 染色發現其分子量大於250kDa。以(N-(3-[2-fury1]acryloy1)-Phe-Gly-Gly)(FAPGG)為酵素反應基質，此一純化的山藥黏質多醣能抑制血管收縮素轉化酶（102.46到409.84 ug/mL，分別抑制28.7%到59.8%血管收縮素轉化酶活性）其效果隨劑量增加而增加。山藥黏質多醣對於血管收縮素轉化酶50%抑制濃度為256.2 ug/mL[對照組captopril為7.81nM(9.5 pmole)]。多醣類的果膠質（102.46，204.92和307.38 ug/mL）對血管收縮素轉化酶並沒有抑制的效果。利用螢光silica TLC或是逆相C18 HPLC檢測FAPGG及其水解產物FAP，結果顯示山藥黏質多醣可以抑制血管收縮素轉化酶。山藥黏質多醣對於血管收縮素轉化酶為混合制，而Michaelis constant 為0.3304 mM
並列摘要
The tuber mucilage of yam (Dioscorea batatas Decne) (YTM) was extracted and purified to homogeneity, which was confirmed by the toluidine blue staining on a sodium dodecylsulfate-polyacrylamide gel electrophoresis gel treated with 2-mercaptoethanol appearing as a single band with molecular mass larger than 250 kDa. This purified YTM was shown by spectrophotometric method to inhibit angiotensin converting enzyme (ACE) in a dose-dependent manner (28.7 to 59.8% ACE inhibition, respectively, by 102.46 to 409.84 µg/mL YTM) using (N-(3-[2-furyl]acryloyl)-P he-Gly-Gly) (FAPGG) as a substrate. The concentration of YTM required for 50% inhibition (IC50) of ACE activity was 256.2 µg/mL while that of captopril was 0.00781 µM (0.0095 nmole). The commercial polysaccharide pectin (102.46 to 307.38 µg/mL) showed no inhibitory activity against ACE. Using fluorescent silica TLC or C18 reverse phase HPLC to detect FAPGG and FAP, the results also showed that YTM inhibited ACE. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health. The YTM showed mixed type inhibition against ACE, and the Michaelis constant in the presence of YTM was 0.33 mM. Consumption of yam tubers may benefit people's health.
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延伸閱讀
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