Fine Mapping of the Nuclear Fertility Restorer Gene for HL Cytoplasmic Male Sterility in Rice

水稻紅蓮型細胞質雄性不育恢復基因（細胞核）的精細定位

黃靖宇(Jing-Yu Huang) ； 胡駿(Jun Hu) ； 徐鑫(Xin Xu) ； 李紹清(Shao-Qing Li) ； 易平(Ping Yi) ； 楊代常(Dai-Chang Yang) ； 任福剛(Fu-Gang Ren) ； 劉學群(Xue-Qun Liu) ； 朱英國(Ying-Guo Zhu)

《Botanical Bulletin of Academia Sinica》 44卷4期 (2003/10) Pp. 285-289

https://doi.org/10.7016/BBAS.200310.0285

摘要

利用群分法，以（從廣41A X 密陽23）X從廣41B回交群體為基因定位群體對紅蓮型細胞質雄性不育恢復基因進行了精細定位。位於10號染色體上的微衛星標記 MIRG4456與紅蓮型細胞質雄性不育恢復基因連鎖，遺傳距離為1.57cM；另一個新發展的微衛星標記HL01被定位於恢復基因的另一端，遺傳距離為0.63cM。這兩個與恢復基因緊密連鎖的微衛星標記有助於對紅蓮型細胞質雄性不育恢復系的分子標記輔助選擇，同時為用圖位克隆法分離紅蓮型細胞質雄性不育恢復基因打下了堅實的基礎。我們也詳細的敘述了利用水稻基因組測系結果發展的微衛星標記的具體方法。

關鍵字

水稻 ； 恢復基因 ； 微衛星標記 ； 標記輔助選擇

並列摘要

Bulked segregant analysis (BSA) of a BC1 population derived from congguang 41A/MiYang 23//congguang 41B was used to map the nuclear fertility restorer gene Rf5 for HongLian (HL) cytoplasmic male sterility. The parents and two bulks representing extremely fertile and sterile plants, respectively, were screened for polymorphism with 20 microsatellite primer pairs on chromosome 10, chosen on the basis of previous research. MRG4456 is linked to the fertility restorer gene Rf5 at a distance of 1.57 cM, and another newly developed microsatellite primer, HL01, was locked at a distance of 0.63 cM to Rf5. Closely linked DNA markers will facilitate not only breeding but also the purity management of hybrid seeds. Concrete steps for developing new microsatellite markers using the rice whole genomic sequence database are also described.

並列關鍵字

Fertility restorer gene ； Marker-assisted selection ； Microsatellite marker ； Oryza sativa L