Thidiazuron Induced High Frequency Shoot Organogenesis in Callus from Kigelia pinnata L.

Thidiazuron誘導紫葳科Kigelia pinnata L.癒傷組織之高頻率的莖器官形成

T. Dennis Thomas ； Jos T. Puthur

《Botanical Bulletin of Academia Sinica》 45卷4期 (2004/10) Pp. 307-313

https://doi.org/10.7016/BBAS.200410.0307

摘要

Kigelia pinnata L.乃紫葳科之一多用途樹種，我們對此發展出一套有效的再生系統。我們在試管內培養節(node)之部份，找出誘導癒傷組織形成之最適植物生長調控素。脆的、具器官形成能力之癒傷組織乃得自節之底切處。當所用之MS培養基輔以3μM 2,4-D的時候具形態潛力的癒傷組織之產率可達100%。此癒傷組織在所用之癒傷誘導基質之前四次繼代培養維持高的再生能力。當MS培養基輔以3μM thidiazuron (N-phenyl N' 1,2,3-thidiazol-5-yl urea) (TDZ)及0.5μM NAA時，可100%再生最大數目之莖(28/culture)，從癒傷組織之多莖形成經組織學方法確認。再生後之莖在½MS培養基輔以4μM indole-3-butyric acid時可達最大發根狀態。我們檢驗 vesicular arbuscular mycorrhizae之附著是否有益減緩移植所導致的衝擊，結果發現非常有幫助：移植60天後仍有100%之存活率。本文所述之高效的再生系統提供生產此多用途樹種之轉殖植物的基礎。

關鍵字

Kigelia pinnata L. ； 藥用植物 ； 植物再生 ； Thidiazuron ； 組織培養 ； Vesicular arbuscular mycorrhizae

並列摘要

An efficient regeneration system was developed for Kigelia pinnata L., a multipurpose tree belonging to the family Bignoniaceae. The nodal segments were cultured in vitro, and the optimum concentrations of plant growth regulators for callus induction were determined. The friable organogenic calli were derived from the basal cut end of the nodal segments. The highest yield of morphogenic callus (l00%) was observed when nodal segments were cultured on Murashige and Skoog (MS) medium supplemented with 3μM 2,4 dichlorophenoxyacetic acid (2,4-D). The morphogenic callus maintained high regeneration during the first four subcultures in the callus induction medium. The maximum shoots (28 culture) were regenerated at the highest frequency of 10000 when 3μM thidiazuron (N-phenyl N' 1,2,3-thidiazol-5-yl urea) (TDZ) and 0.5μM naphthaleneacetic acid (NAA) were added to MS medium. The emergence of multiple shoots from the calli was histologically documented. The regenerated shoots showed maximum rooting on ½MS medium containing 4μM indole-3-butyric acid (IBA). The effect of vesicular arbuscular mycorrhizae (VAM) association in averting the transplantation shock was tested and proved to be highly beneficial, giving a 100% survival rate after 60 d of transplantation. This efficient plant regeneration system provides a foundation for generating transgenic plants of this multipurpose tree.

並列關鍵字

Kigelia pinnata L. ； Medicinal plant ； Plant regeneration ； Thidiazuron ； Tissue culture ； Vesicular arbuscular mycorrhizae