Variation and Segregation Following Nuclear Transplantation in Antrodia cinnamomea

牛樟芝細胞核轉殖後所產生的變異及分離

王威閔(Wei-Ming Wang) ； 吳瑞鈺(Rey-Yuh Wu) ； 柯文雄(Wen-Hsiung Ko)

《Botanical Bulletin of Academia Sinica》 46卷3期 (2005/07) Pp. 217-222

https://doi.org/10.7016/BBAS.200507.0217

摘要

細胞核轉殖的牛樟芝(Antrodia cinnamomea)原生殖體，再生之後多數呈現出與親本不同的外表型態，可認定為細胞核雜交體。細胞核轉殖所獲得到的雜交體外表型態呈現不穩定，並且普遍有扇型菌落的產生。多數的雜交體呈現生長緩慢，這些雜交體經由繼代培養之後仍持續正常生長。細胞核雜交體超過一半有恢復成親本原生質體再生的菌落型態，這結果顯示粒線體基因對於菌株生理可能扮演一個重要的角色，菌落的外表型態是由於細胞核與粒腺體基因之間的交互作用所產生。

關鍵字

牛樟芝 ； 細胞核雜交體 ； 細胞核轉殖

並列摘要

Intraspecific fusion of protoplasts with nuclei in Antrodia cinnamomea lengthened the time for development of colonies on regeneration medium and reduced the number of protoplasts capable of regeneration. When 2.1×10^5 C98 protoplasts were fused with C96 nuclei, 1750 colonies appeared on the regeneration medium, and 742 of them were putative nuclear hybrids as their colonies were different from that of the protoplast parent. Without the presence of C96 nuclei, 3160 colonies appeared on the regeneration medium and 400 of them tested were similar to the protoplast parent in colony appearance. No colonies appeared when self-fused nuclei were plated on regeneration medium. The rate of successful transfer was 23%. The success rate was for reciprocal transfer. About 77 to 82% of nuclear hybrids were slow in growth. These isolates resumed normal growth upon subculture. All the nuclear hybrids obtained were unstable. Segregation occurred during growth of mycelia. resulting in the formation of colonies different from those of the originals in subcultures. After five consecutive subcultures, 13 stable nuclear hybrids were obtained. These isolates differed greatly from the parental isolates and from each other in pigmentation, growth rate, and colony pattern.

並列關鍵字

Antrodia cinnamomea ； Colony morphology ； Nuclear hybrid ； Nuclear transplantation