水稻白葉枯病抗病檢定方法之改良

Improvement of Resistant Screening Techniques for Bacterial Leaf Blight of Rice Caused by Xanthomonas oryzae pv. oryzae

謝麗娟(Lih-Jiuan Hsieh) ； 張義璋(Yih-Chang Chang) ； 謝廷芳(Ting-Fang Hsieh)

《台灣農業研究》 54卷1期 (2005/03) Pp. 15-22

https://doi.org/10.6156/JTAR/2005.05401.02

摘要

本研究為探討水稻白葉枯病抗病檢定接種技術，以四個供試稻品種，於1994年第二期作及1995年第一期作，測定30、45和60天苗期，孕穗期及抽穗期等生育期之抗感病性反應。結果顯示任一稻品種以剪葉法接種，都以30天苗期之平均罹病指數為最高，且除抽穗期外，其它各生育期接種均能檢定出稻品種間不同之抗感病性，又45及60天苗期稻株接種之結果，與本田孕穗期檢定之結果相近。另外，於抽穗期測定剪葉長度及不同葉位序剪葉接種對發病之影響，結果任一稻品種以剪除1/2、1/4及1/8葉長度間之平均罹病指數並無顯著差異，且1/2剪葉長度接種，除第一期作稻外，都能檢測出不同品種之抗感病性。再以1/2剪葉長度接種劍葉以下四個位序葉片，發現不同稻品種不同葉位序間之平均罹病指數差異變化大，但大多以第四位序為最高，且除了第一期作稻外，接種第一和第二位序時，較可區別出品種間不同之抗感病性。

關鍵字

水稻 ； 水稻白葉枯病 ； 抗病性

並列摘要

The objectives of this study were to evaluate the susceptibility of rice cultivars at different growth stages to bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae by the leaf clipping inoculation method and to determine effects of clipping off leaves at different lengths and different positions on the disease reaction. The experiments were conducted at the second crop of 1994 and the first crop of 1995 in a greenhouse and in the Institute's experimental field. Inoculation of plants at 30, 45 and 60 days after sowing (DAS), and at booting and heading stages of four rice varieties including two indica (Tainung sen 18, resistant and Tainung sen 3, susceptible) and two japonica (Tainung 61, resistant and Tainung 67, susceptible) rice showed that the 30 DAS stage was the most susceptible to infection of the bacterial pathogen in each variety. Except the heading stage, the inoculation at all growing stages could distinguish the resistant and susceptible varieties. This suggests that the result of resistant screening at 45 or 60 DAS stage was similar to that normally evaluated at the booting stage in Taiwan. At the heading stage, there was no significant difference in disease severity of a rice variety among inoculations by clipping off half, one-fourth, and one-eighth length of leaf. However, the inoculation by cutting half length of leaf could evaluate the disease resistance among the four rice varieties, except the japonica variety in the first crop trials. In addition, the inoculation method with cutting half length of leaf on the 1st and 2nd leaf from top could consistently distinguish the disease resistance of the rice varieties, except the japonica variety in the first crop trials.

並列關鍵字

Rice ； Bacterial leaf blight of rice ； Resistance screening techniques