利用RAPD與ISSR分子標誌鑑定西瓜品種

Molecular Markers Derived from RAPD and ISSR Analysis for Identification of Watermelon

王昭月(Jau-Yeuh Wang) ； 張有明(You-Ming Chang) ； 沈百奎(Bor-Kwei Shen) ； 王毓華(Yu-Hua Wang) ； 劉邦基(Pan-Chi Liou)

《台灣農業研究》 54卷4期 (2005/12) Pp. 257-269

https://doi.org/10.6156/JTAR/2005.05404.06

摘要

農業試驗所新育成之西瓜新品種：台農六號（2003年）、台農七號（2004年），以及經濟栽培種合計八個品種（系），利用RAPD(random amplified polymorphic DNA)與ISSR(inter simple sequence repeat)兩種分子標誌，進行不同品種指紋分析與品種間遺傳相似性之評估。試驗結果可自9個具再現性的RAPD引子反應，產生127個RAPD條帶(band)，其中93個標誌條帶具多型性，多型性百分率為73.2%；在ISSR分析中，18個引子可產生198個條帶，其中120個為多型性標誌條帶，多型性百分率為60.6%。針對八個栽培種之指紋分析，共篩選獲得24個RAPD標誌條帶，以及37個ISSR標誌條帶，可將不同栽培品種（系）完全鑑別。進一步將兩種分子標誌進行UPGMA(un-weighted pair-group mean arithmetic)之遺傳相似性分析，結果顯示以RAPD或ISSR分子標誌計算之遺傳相似性樹狀圖具有相近之趨勢。據ISSR分子標誌的Dice相似性係數估算，3個經濟栽培種同群，其遺傳相似性係數為0.58；農業試驗所新育成之西瓜台農六號、台農七號及其親本，則明顯另成一群，其遺傳相似性係數為0.44；連鎖此兩群之遺傳相似性係數為0.34。綜合觀之，RAPD或ISSR兩種分子標誌均可應用於西瓜品種鑑定，皆為有效之指紋分析工具。

關鍵字

西瓜 ； 指紋分析 ； 逢機增殖多型性DNA ； 間帶簡單序列重複

並列摘要

RAPD and ISSR markers were obtained for eight watermelon [Citrullus lanatus (Thunb) Mansf.] cultivars (lines) including two hybrids [Tainung No. 6 and Tainung No. 7 bred at Agricultural Research Institute (ARI)] for fingerprinting and assessment of their genetic relationship. Nine reproducible RAPD primers generated 127 fragments, among which 93 were polymorphic bands (polymorphism 73.2%). Eighteen ISSR primers yielded 198 ISSR fragments, of which 120 were polymorphic bands (polymorphism 60.6%). Fingerprinting analysis showed that 24 genotype specific RAPD markers and 37 specific ISSR markers were sufficient to differentiate these cultivars (lines). The genetic relationship and genetic similarity between cultivars (lines) were estimated using Dice coefficient of similarity of all of the ISSR or RAPD markers. Based on UPGMA (un-weighted pair-group mean arithmetic), the pedigree information and the dendrogram of these two markers were similar. Analysis of the similarity coefficient of ISSR markers revealed that three commercial cultivars with a genetic similarity of 0.58 belonged to a cluster different from the cluster of two hybrids with genetic similarity 0.44. These two groups had genetic similarity coefficient value of 0.34. It is suggested that both RAPD and ISSR could be utilized as a reliable tool for the fingerprinting and assessment of the genetic relationship of watermelon cultivars.

並列關鍵字

Watermelon ； Fingerprinting ； RAPD random amplified polymorphic DNA ； ISSR inter simple sequence repeat