銀耳多醣應用為皮膚保養原料之活體外及活體內評估

In Vitro and in Vivo Evaluations of Tremella Polysaccharides for Skin Care

楊淑惠(Shu-Hui Yang) ； 楊鎮榮(Jenn-Rong Yang) ； 陳宜嫻(Yi-Shyan Chen)

《台灣農業研究》 56卷2期 (2007/06) Pp. 143-151

https://doi.org/10.6156/JTAR/2007.05602.07

摘要

本研究探討銀耳多醣應用於皮膚保濕用途之可行性，並進行安全性評估。本實驗所使用之銀耳多醣重量平均分子量為2,000-5,000 Kdalton。安全性試驗顯示，銀耳多醣在濃度250 μg/mL下不會抑制STO小鼠胎體層纖維細胞正常生長，經連續14 天長期培養對其膠原蛋白生合成有部份促進效果。銀耳多醣不會導致L929細胞變形，無細胞毒性；對供試動物皮膚刺激指數為零，表示對皮膚無刺激性。對人體皮膚經180分鐘的保濕功效評估顯示，銀耳多醣溶液能提高正常皮膚角質層35-56%的含水率，可提高乾燥皮膚角質層25-44%含水率，故銀耳多醣為安全之天然保濕原料，且無刺激性。

關鍵字

銀耳 ； 多醣體萃取液 ； 皮膚細胞毒性 ； 保濕

並列摘要

The fruiting body of Tremella fuciformis Berk is a famous traditional food in Taiwan and China. The polysaccharides extracted from T. fuciformis have been reported to display several pharmacological activities. In this study, the moisturizing effect of Tremella polysaccharides were studied and its safety for use as a novel application of skin care was evaluated. Result showed that the molecular weight of Tremella polysaccharides is in between 2,000,000 and 5,000,000 dalton. The STO mouse embryonic fibroblast cells grew normally and its collagen synthesis increased when the culture medium was amended with 5-250 μg/mL Tremella polysaccharides. Tremella polysaccharides didn't cause malformation of tested L929 cells of mouse fibroblasts. The primary irritation index was zero and the overall response felled into the category 'negligible' for skin irritation test. When applying to skin for 180 minutes, the Tremella polysaccharides increased the ratio of the water content in the stratum corneum about 35-56% for normal skin and 25-44% for dry skin, respectively. This indicates that Tremella polysaccharides are safe for the development of a moisturizing product for skin care.

並列關鍵字

Tremella fuciformis ； Polysaccharides ； Cells toxicity ； Moisturizing product