ABA、Fluridone及Sucrose對鐵炮型及群間雜交種百合鱗片葉發育形態影響之研究

The Effects of ABA, Fluridone and Sucrose on the Scale Leaves Morphogenesis of Longiflorum Type and Intra-hybrid Lilies

戴廷恩(Ting-En Dai) ； 許圳塗(Chou-Tou Shii)

《台灣農業研究》 58卷3期 (2009/09) Pp. 234-242

https://doi.org/10.6156/JTAR/2009.05803.08

摘要

爲研究ABA對於百合鱗片葉發育形態之影響，以台灣百合(F)、鐵炮百合(L)、東方型百合栽培種'Casa Blanca' (CA)及群間雜交種F×CA及L×CA爲材料，利用ABA、Fluridone及糖於試管內進行研究。外加ABA及Fluridone培養4週及8週，鱗片葉生長調查結果顯示鱗片葉生長均明顯受到ABA的抑制及Fluridone的促進，ABA會抵銷Fluridone的效應。ABA濃度變化結果顯示CA有最高內生ABA濃度，F及L最低，雜交種則介於兩親本間，外加ABA促使鱗片ABA濃度增加，Fluridone處理使鱗片ABA內含量濃度減少。糖對鱗片葉形態發育的調查數據顯示高濃度糖抑制鱗片葉的發生，但抑制效果不如外加ABA，依然有一定程度的鱗片葉發生。

關鍵字

百合 ； 離層酸 ； 離層酸合成抑制劑 ； 蔗糖 ； 鱗片葉

並列摘要

To investigate the effects of exogenous ABA on the development of scale leaf, four different clones of L. formosanum (F), L. longiflorum (L), oriental hybrid 'Casa Blanca' and related distant hybrids (F×CA, L×CA) were in vitro cultured under different ABA, Fluridone and sugar concentration. Four and eight weeks after culture, it showed that the growth of scale leaves among all genotypes were promoted by ABA but inhibited by Fluridone, ABA can diminish the effects of Fluridone, and Fluridone treatment increases the number of scale leaves but somewhere bleaching in leaf blade. In applied ABA or Fluridone, L/O hybrids showed intermediate performance as compared with F and L populations. Four weeks after culture, un supplement both ABA and Fluridone, L/O hybrids had better scale leaf growth than their parents. Based on the analysis of ABA concentration, it showed that the endogenous of ABA concentration was the highest in CA, lowest in F and L, and L/O hybrids were intermediate, exogenous ABA stimulates endogenous ABA synthesize in the scale, Fluridone inhibits endogenous ABA concentration in the scale, and exogenous ABA can diminished the effects of Fluridone. The effects of sucrose on the scale leaf morphogenesis showed that higher sucrose concentration inhibits the development of scale leaves. However, this effect of inhibition was less than exogenous ABA, since scale leaf emergence was still observed in sucrose treatment. In additionally, high concentration sucrose combined with Fluridone can promote the scale leaf development. Whatever supplement Fluridone or not in the medium, the effects of high concentration sucrose showed no significant change on endogenous ABA in scale of F, L and CA but increase in L/O hybrids.

並列關鍵字

Lily ； ABA ； Fluridone ； Sucrose ； Scale leaf