- 期刊
Pseudomonas viridiflava 引起之番茄髓壞疽病
Occurrence of Pith Necrosis of Tomato Caused by Pseudomonas viridiflava in Taiwan
摘要
2011 年於南投縣與花蓮縣地區少數番茄栽植田，發現部分植株出現生長緩慢及衰弱情形，病株莖部表面出現暗褐色塊狀病斑，縱切莖部發現其髓部組織出現褐色壞疽，病勢嚴重時整株萎凋。從罹病莖部壞疽組織分離純化出1 種病原細菌，該細菌經接種番茄植株證實可引起相同病徵，並完成科霍氏法則，確認為該細菌所引起。該細菌經生理生化特性、Biolog 系統測試及16S rDNA 序列分析，鑑定出此細菌為Pseudomonasviridiflava，此為P. viridiflava 在台灣造成番茄細菌性髓壞疽病之首次報導。以濾紙圓盤擴散法測試市售10 種藥劑對該病原菌的抑制圈大小，結果顯示在3 種有效測試濃度 (ai) 下對病原菌之抑制生長效果，以歐索林酸最佳。
並列摘要
In 2011 some infected tomato plants were noticed in several fields of Nantou and Hualien Counties. Dark brown blotch was observed in the surface of stem. Internally, pith and vascular tissues showed brown discoloration and necrosis symptom. The pathogenicity of the bacteria isolated from necrotic stem tissues was verified by Koch’s postulates. The pathogen was further identified as Pseudomonas viridiflava based on physiological and chemical tests, Biolog identification, and 16S rDNA sequence analysis. This is the first report of pith necrosis of tomato caused by P. viridiflava in Taiwan. On screening the agrochemicals, oxolinic acid was the most efficient to inhibit the pathogen growth.
延伸閱讀
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