In this study, for comparing different levels of tolerance in banana species to Fusarium wilt disease, several cultivars, i.e., 'Pei Chaio', 'Formosana', 'Tai Chiao No. 5', 'Gros Michel', 'Pisang Awak', and 'Rose', which were commonly cultivated in Taiwan, were inoculated artificially with Fusarium pathogen. Percentages of chlorosis were estimated after culturing two-month-old seedlings of bananas in Fusarium-inoculated soil in greenhouse. Besides, we examined browning region of corm sections of Foc-inoculated plants quantitatively and qualitatively and estimated the amounts of Foc in each corm section by real-time PCR method. Results showed that cultivar 'Rose' was the more resistant cultivar among six cultivars while 'Gros Michel' was the more susceptible cultivar.