Effect of Epidermal Growth Factor (EGF) on Tetrahymena pyriformis

László Köhidai ； Márk Keresztesi ； György Csaba

《Acta Protozoologica》 40卷3期 (2001/09) Pp. 221-224

並列摘要

Tetrahymena has receptors for hormones characteristic in higher animals and signal transduction systems for provoking response. In the present experiments EGF, a potent mitogen of epithelial cells were studied. In a 24 h experiment 1 h treatment with different concentrations of EGF (0.1-10 µg) significantly decreased the growth of Tetrahymena. Five microgram EGF treatment 24 h after 5 µg EGF pretreatment (imprinting) also significantly reduced the amount of cells, and this was moderately, however significantly less, than without the repeated treatment. After 48 h there was not difference between the amount of single or double treated cells. EGF was chemorepellent in chemotaxis experiments and 4 h EGF pretreatment abolished this effect, possibly by down regulation. The experiments call attention to the effect of EGF at a very low phylogenetic level, however the direction of the effect was the opposite of the effect in higher animals.

並列關鍵字

cell growth ； growth factors ； chemotaxis ； evolution ； Tetrahymena