Karyotype and Genome Size of Nadelspora canceri Determined by Pulsed Field Gel Electrophoresis

Harold P. Amogan ； J. Patrick Martinez ； Lynda M. Ciuffetti ； Katharine G. Field ； Paul W. Reno

《Acta Protozoologica》 45卷3期 (2006/09) Pp. 249-254

並列摘要

Pulsed field gel electrophoresis was used to characterize the genome of microsporidian Nadelspora canceri. N. canceri spores isolated from Dungeness crab (Cancer magister) and red rock crab (Cancer productus) both yielded a karyotype of 10 chromosome-sized DNA bands. Close inspection of the band patterns and band sizes showed a difference in genome size between the two isolates. Spore isolates from Dungeness crab had a genome size of 7.4 Mb while spore isolates from red rock crab had a genome size of 7.3 Mb. Previously characterized microsporidians have a genome size range of 2.9-19.5 Mb and a karyotype ranging from 7-18 chromosome-sized DNA bands. N. canceri falls well within the genome size range and karyotype number of known microsporidians. The difference in genome size observed between the two spore isolates indicates intraspecies chromosome-size polymorphism.

並列關鍵字

chromosome-size polymorphism ； microsporidia ； protists