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Excystment-Inducing Factors in the Ciliated Protozoan Colpoda cucullus: Hydrophobic Peptides Are Involved in Excystment Induction
並列摘要
Indispensable elements such as nutrients contained in plant extracts for the survival and growth of the vegetative cells of protozoans and their food organisms, i.e. bacteria are possibly the signal for excystment induction. In the present study, therefore, the excystment-inducing effects of nutrients (vitamins, sugars, amino acids, polypepton, BSA) were examined on the resting cysts of Colpoda cucullus. Among the tested nutrients, only polypepton (mixture of peptides and amino acids obtained by protease digestion of casein) showed a marked excystment-inducing activity. The excystment-inducing activity of polypepton was fractionated by a series of chromatography (gelfiltration chromatography, normal-phase HPLC and normal-phase TLC), and the activity was finally isolated in spots containing relatively hydrophobic peptides in the TLC. It is possible that certain common structures contained in such peptides having the excystment-inducing activity may be widely present in the plant extract.
延伸閱讀
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