- 期刊
- OpenAccess
Morphology and SSU rRNA Gene Sequences of Three Marine Ciliates from Yellow Sea, China, Including One New Species, Uronema heteromarinum nov. spec. (Ciliophora, Scuticociliatida)
並列摘要
The morphology, infraciliature, and silverline system of three marine scuticociliates, Uronema marinum Dujardin, 1841, U. heteromarinum nov. spec. and Pleuronema setigerum Calkins, 1902, isolated from coastal waters off Qingdao, China, were investigated using living observation and silver impregnation methods. Due to the great confusion in the species definition of the well-known species U. marinum, we have documented a detailed discussion/comparison and believe that most of the confusion is due to the fact that at least 2 closely-related sibling morphotypes exist which are often not recognized. Based on the data available, U. marinum is strictly defined as follows: marine Uronema ca. 30×10 μm in size, with truncated apical frontal plate and smooth pellicle, extrusomes inconspicuous, cytostome located equatorially, 12-14 somatic kineties and one contractile vacuole pore near posterior end of kinety 2. Uronema heteromarinum nov. spec. resembles U. marinum but can be distinguished morphologically by its notched pellicle with conspicuous extrusomes and reticulate ridges, the 15-16 somatic kineties, widely separated membranelle 1 and membranelle 2, as well as the subequatorially positioned cytostome. Based on the Qingdao population, an improved diagnosis for the poorly known Pleuronema setigerum is: marine slender oval-shaped form, in vivo about 40-50×15-20 μm; 3-5 preoral kineties and 14-22 somatic kineties; membranelle 1 and 3 three-rowed, and posterior end of M2a ring-like. The small subunit (SSU) rRNA gene for all three organisms were sequenced and analyzed with standard methods.
延伸閱讀
- Shang, H. M., Song, W. B., & Warren, A. (2003). Phylogenetic Positions of Two Ciliates, Paranophrys magna and Mesanophrys carcini (Ciliophora: Oligohymenophorea), within the Subclass Scuticociliatia Inferred from Complete Small Subunit rRNA Gene Sequences. Acta Protozoologica, 42(3), 171-181. https://www.airitilibrary.com/Article/Detail?DocID=00651583-200309-201012290010-201012290010-171-181
- Mazei, Y., Gao, S., Warren, A., Li, L. Q., Li, J. Q., Song, W. B., & Esaulov, A. (2009). A Reinvestigation of the Marine Ciliate Trachelocerca ditis (Wright, 1982) Foissner and Dragesco, 1996 (Ciliophora, Karyorelictea) from the Yellow Sea and an Assessment of Its Phylogenetic Position Inferred from the Small Subunit rRNA Gene Sequence. Acta Protozoologica, 48(3), 213-221. https://www.airitilibrary.com/Article/Detail?DocID=00651583-200909-201101060033-201101060033-213-221
- LIU, M., LI, L., QU, Z., LUO, X., FARRAJ, S. A. A., LIN, X., & HU, X. (2017). Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis. Acta Protozoologica, 56(1), 17-37. https://doi.org/10.4467/16890027AP.17.003.6967
- Jung, J. H., Park, K. M., & Min, G. S. (2014). Morphology and Molecular Phylogeny of Pseudouroleptus jejuensis nov. spec., a New Soil Ciliate (Ciliophora, Spirotrichea) from South Korea. Acta Protozoologica, 53(2), 195-206. https://doi.org/10.4467/16890027AP.14.016.1597
- PAN, X. M., Huang, J., Fan, X. P., Ma, H. G., RASHEID, K. A. S. A., MIAO, M., & Gao, F. (2015). Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.. Acta Protozoologica, 54(1), 31-43. https://doi.org/10.4467/16890027AP.15.003.2190