野黃芩素抑制人類肺癌細胞株增生的分子機轉

The Molecular Mechanism of Scutellarein Inhibits Human Lung Cancer Cells Proliferation

高而仕(Erl-Shyh Kao)

《中西整合醫學雜誌》 15卷3期 (2013/09) Pp. 23-28

摘要

人類肺癌細胞株A549的增生速度快，而其中具有主導細胞增殖的環氧合酶2（cyclooxygenase-2，COX2）大量表現是主要因素之一。野黃芩素（scutellarein）是一種類黃酮分子（flavonoids），具有抗發炎與抗氧化的功效。實驗測試含有不同濃度（0，5，25，50μM）野黃芩素的培養基與A549細胞培養48小時，發現50μM野黃芩素培養基可以使A549細胞增生的速率減緩40%以上。進一步的蛋白質表現量分析發現野黃芩素可以抑制人類肺癌細胞株A549增生能力，主要是透過抑制表皮生長因子受體（epidermal growth factor receptor，EGFR）減少細胞外調節蛋白激酶（extracellular regulated proteinkinases，ERK）的磷酸化，進一步減少核轉錄因子（nuclear factor κB，NFκB)蛋白的表現，最後抑制了COX2的產生，而阻止了A549細胞的增生。

關鍵字

A549細胞株 ； 野黃芩素 ； 環氧合酶2

並列摘要

Human lung cancer cell line A549 has a strong ability in proliferation. Cyclooxygenase-2, which can induce hyperplasia in the cells, highly expression is one of the major reasons. Scutellarein is a compound, as flavonoids, with anti-oxidant and anti-inflammatory activity. In this work, we screened the various concentrations (0, 5, 25,50μM) of scutellarein in medium and cultured with A549 cell line for 48h. After the incubation with 50μM scutellarein for 48h, A549 cell line proliferation ability decreased more than 50%. Further, the protein analysis discovered that the growth inhibition of A549 was administrated by epidermal growth factor receptor (EGFR) pathway. Scutellarein treatment can decrease extracellular regulated protein kinases (ERK) activation , and decreased the expression of nuclear factor κB (NFκB). Finally, scutellarein treatment inhibited the COX2, which is the down stream target of EGFR pathway, and inhibited the proliferation of A549 cell line.

並列關鍵字

A549 cellline ； scutellarein ； cyclooxygenase-2