野生狼尾草種原RAPD標誌之群叢分析

Cluster Analysis of Germplasms of "Pennisetum purpureum" with RAPD Markers

林正斌(Jeng-Bin Lin) ； 李姿蓉(Tzu-Rung Lee) ； 林明村(Ming-Tsun Lin) ； 蘇建安(Cheng-Zan Sung) ； 侯金日(Chin-Jin Hou)

《中華民國雜草學會會刊》 32卷1期 (2011/06) Pp. 23-33

https://doi.org/10.6274/WSSROC-2011-032(1)-023

摘要

Napiergrass (Pennisetum purpureum) is one of the most important forage crops in Taiwan. The hybrid seeds of napiergrass spread widely. It causes diverse germplasms of napiergrass in the field. In this experiment, 104 samples of native napiergrass germplasms were collected from 15 areas in Taiwan. The genetic diversity of these napiergrass germplasms were determined by random amplified polymorphic DNA (RAPD) analysis. The results showed that 94.18% (p＜0.001) variance within the population was significantly different by analysis of molecular variance (AMOVA). The hereditary-distance was ranged from 0.0003 to 0.3 among the 15 areas. With the herediary-distance of 0.03, the 104 napiergrass germplasms could be divided into 7 groups: the 1st group from Taipei and Hsinchu, the 2nd group from Miaoli, the 3rd group from Taoyuan, the 4th group from Taitung and Pingtung, the 5th group from Taichung, Nantou, Hualien, Changhua and Yunlin, the 6th group from Tainan, Chaiyi and Kaohsiung, and the 7th group from Eilan. The correlation coefficient between the genetic distance and the cluster dendrogram was 0.68. Taken together, the results suggested that napiergrass lines from the 7 groups might be sufficient for further evaluation to select the elite lines within a shorter period.

關鍵字

狼尾草 ； 種原 ； 逢機增殖多型性 ； 群叢分析

並列摘要

Napiergrass (Pennisetum purpureum) is one of the most important forage crops in Taiwan. The hybrid seeds of napiergrass spread widely. It causes diverse germplasms of napiergrass in the field. In this experiment, 104 samples of native napiergrass germplasms were collected from 15 areas in Taiwan. The genetic diversity of these napiergrass germplasms were determined by random amplified polymorphic DNA (RAPD) analysis. The results showed that 94.18% (p＜0.001) variance within the population was significantly different by analysis of molecular variance (AMOVA). The hereditary-distance was ranged from 0.0003 to 0.3 among the 15 areas. With the herediary-distance of 0.03, the 104 napiergrass germplasms could be divided into 7 groups: the 1st group from Taipei and Hsinchu, the 2nd group from Miaoli, the 3rd group from Taoyuan, the 4th group from Taitung and Pingtung, the 5th group from Taichung, Nantou, Hualien, Changhua and Yunlin, the 6th group from Tainan, Chaiyi and Kaohsiung, and the 7th group from Eilan. The correlation coefficient between the genetic distance and the cluster dendrogram was 0.68. Taken together, the results suggested that napiergrass lines from the 7 groups might be sufficient for further evaluation to select the elite lines within a shorter period.

並列關鍵字

Pennisetum purpureum ； germplasm ； RAPD ； cluster analysis