利用ISSR分子標誌檢測臺灣紫色狼尾草（Pennisetum purpureum Schum）遺傳歧異度

Determining the Genetic Diversity of Purple Napiergrass with ISSR Markers in Taiwan

陳皇丞(Huang-Cheng Chen) ； 林明村(Ming-Tsun Lin) ； 李姿蓉(Tzu-Rung Li) ； 侯金日(Chin-Jin Hou) ； 林正斌(Jeng-Bin Lin)

《中華民國雜草學會會刊》 33卷2期 (2012/12) Pp. 77-90

https://doi.org/10.6274/WSSROC-2012-033(2)-077

摘要

紫色狼尾草（Pennisetum purpureum Schum）為台灣自生草種，本研究利用簡單序列重複區間（inter simple sequence repeat, ISSR）分子標誌，分析由臺灣各地收集到之紫色狼尾草收集系之遺傳結構。材料逢機收集自臺灣15縣市共71樣本，使用購自UBC（University of British Columbia）之15組具多型性ISSR引子進行PCR擴增分析，結果獲得205條核酸條帶，其中多型性條帶有101條（佔49.26%）。經由POPGENE分析，總基因歧異度（H）為0.2215，族群間之分化係數（Gst）為0.4205，基因流（Nm）為0.6892。由分子變方分析（AMOVA）結果，族群間之變方成分為7.55%，族群內之變方成分為92.45%，顯示族群間並無較明顯分化趨勢，主要之遺傳變異存在於族群內個體間。依遺傳距離矩陣與地理距離矩陣之相關性測驗（Mantel test），顯示二者並無顯著關聯性（r = 0.1137，p＜0.5452）。依歸群與主座標分析（Principal coordinate analysis, PCoA）結果，於遺傳距離0.04位置可將15縣市之地理族群區分為5群，分別為：第1群：台北、桃園、新竹、苗栗、台中、南投、嘉義及台南；第2群：宜蘭；第3群：彰化及雲林；第4群：高雄、屏東及台東；第5群：花蓮。爾後可依此研究結果，搭配各收集系間之農藝性狀及成份差異進行選拔，藉以縮短育種選拔時間。

關鍵字

紫色狼尾草 ； 簡單序列重複區間 ； 群叢分析

並列摘要

Purple napiergrass (Pennisetum purpureum Schum) is a native grass species in Taiwan. In this study 71 samples collected from 15 counties in Taiwan were used and ISSR marker from UBC (University of British Columbia) was used to determine the genetic structure. PCR amplification with 15 ISSR primers yielded 205 DNA fragments, and 101 of these DNA fragments were polymorphic (49.26 %). POPGENE analysis revealed total gene diversity (H), genetic differentiation index (Gst) and gene flow (Nm) were 0.2215, 0.4205 and 0.6892, respectively. Analysis of molecular variance (AMOVA) revealed the variance component among and within populations were 7.55 % and 92.45 %, respectively. This result showed that no significant variation in diversity was observed among populations. The major genetic differentiation was due to variation within populations. In addition, there was no significant relation between genetic distance and geographic distance (r = 0.1137, p＜0.5452) . Both the UPGMA cluster analysis and principal coordinate analysis (PCoA) showed that 71 samples tested could be divided into five groups by 0.04 genetic distance; the first group includes Taipei, Taoyuan, Hsinchu, Miaoli, Taichung, Nantou, Chiayi and Tainan, the second group includes Yilan, the third group includes Changhua and Yunlin, the fourth group includes Kaohsiung, Pintung and Taitung, and the last one includes Hualian. In the future, we can select the elite lines within these five groups by cluster analysis method. It might be helpful to shorten breeding period.

並列關鍵字

Pennisetum purpureum ； ISSR ； cluster analysis