台灣省乳牛無乳鏈球菌特異血清型的種類及分佈

Type-Specific-Serovars Distribution of Streptococcus Agalactiae Isolated from Taiwan

程博文(Bou-Wen Tsung) ； 張登欽(Tung-Ching Chung) ； 楊忠亮(Chung-Liang Young) ； 董好德(Hou-Der Dong) ； 吳春利(Chwein-Li Wu)

《中華民國獸醫學會雜誌》 21卷4期 (1995/08) Pp. 232-241

https://doi.org/10.7009/JTCSVS.199508.0232

摘要

本試驗先以捷克CNCTC（Czeckoslovak National Collecion of Culture）標準菌株免疫兔子並行交叉吸收後，製得9種特異性血清型（type-specific serovars; TSS）血清；並於民國80年7月至81年7月間，由本省北中南三地10個酪農專業區之122個乳牛場，分離得178株無乳鏈球菌（Streptococcus agalactiae; SA）。經以自製的各型血清加以鑑定分析役，得知（一）TSS複合型的分佈率依序分別為：X（42.13%）、IaX（35.96%）、Ia（6.74%）、Ⅱ（2.25 %）、IcX（1.69%）、Ia/c（1.12%）、IaⅡ（0.56%）及Ic（0.56%），無法鑑定血清型（NT）者有5.62%，（二）TSS單型分佈率，最高者為X（83.15%），其次分別是Ia（44.38%）、Ⅱ（6.18）及Ic（3.37%），Ib、Ⅲ、Ⅳ、Ⅴ及R型則未出現，（三）本省三區中，以南區出現的TSS型別種類最多，尤其柳營專業區最為嚴重有8種綜合型出現；當中之IaⅡ、Ic、Ⅱ及ⅡX等4型，更是只出沒於南區，而未曾見於北中2區，（四）中部福寶專業區雖又有5種綜合型，但當中的Ia/c型則為全省所僅見。本試驗所進行之SA菌TSS型血清製備及全省菌株分佈率調查，為本省人醫及獸醫兩方面的第一次。

關鍵字

牛乳房炎 ； 無乳鏈球菌 ； 特異性血清型

並列摘要

By using 10 CNCTC type strains of Streptococcus agalactiae (SA), each with its characteristic type-specific-serovar (TSS), to immunize rabbit and also following with cross-absorption procedure, we succeeded in preparing 9 kinds of TSS sera. We also conducted a wide range investigation regarding the infection rates of SA among 10 major dairy-raising districts located at 3 areas (northern, middle and south) of Taiwan from July 1991 till July 1992. Totally 178 SA strains were isolated from 122 dairy farms during this investigation. TSS type-identification of these 178 local SA strains revealed that: (1) distribution rates of complex-TSS types were X, 42.13%; IaX, 35.96%; Ia, 6.74%; ⅡX, 3.37%; Ⅱ, 2.25%; IcX, 1.69%; Ia/c, 1.12%; IaⅡ, 0.56%; and Ic, 0.56%, (2) distribution rates of mono-TSS types were X, 83.15%; Ia, 44.38%; Ⅱ, 6.18%; and Ic, 3.37%. No strains with type Ib, Ⅲ, Ⅳ, Ⅴ, or R were found in this regard, (3) southern area of Taiwan was the most seriously contaminated one, especially for the Liu-Yin district, where 8 complex-TSS types were identified. Among these 8 complex-TSS tpes, 4 types including Ia Ⅱ, Ic, Ⅱ and ⅡX were even found to be concentrated at this area only, (4) Although as less as only 5 complex-TSS types were discoverd at Fu-Bou district of middle area, one of these complex-TSS types, namely Ia/c, was also found to be unique at this area only. This is the first report regarding with TSS sera preparation and their distribution rates of SA strains isolated from Taiwan.

並列關鍵字

Bovine mastitis ； Streptococcus agalactiae ； Type-specific-serovar