1993至1995臺灣地區雞傳染性支氣管炎病毒之分離及馴化

Isolation and Adaptation of Infectious Bronchitis Virus in Taiwan from 1993 to 1995

曾正昌(Cheng-Chung Tseng) ； 黎南榮(Nan-Zon Li) ； 姚中慧(Chung-Hwei Yao) ； 王金和(Ching-Ho Wang)

《中華民國獸醫學會雜誌》 22卷2期 (1996/04) Pp. 113-120

https://doi.org/10.7009/JTCSVS.199604.0113

摘要

本實驗目的包括分離及鑑定臺灣地區雞傳染性支氣管炎（IB）病毒，並分析目前臺灣IB的發生狀況，對臺灣分離毒株以雞胚腎細胞加以馴化。從1993年至1995年間本研究室共由21場發生場分離出21株IB病毒，發生時間集中於寒冷低溫時節，發生雞齡集中於4-5週齡，發生雞種多為肉雞；合併感染主要為大腸桿菌（E. coli）。發病雞場中21場有13場經IB疫苗免疫。剖檢病變以氣管黏液增加及腎臟尿酸鹽沉積為主。病毒經雞胚胎接種及電子顯微鏡觀察鑑定為IB病毒。與以前分離者合計24株臺灣分離毒株及5株標準毒株經由雞胚腎細胞2-6次繼代，臺灣分離毒株中有6株可形成細胞病理變化（CPE），標準株5株全可形成CPE；可形成CPE者才可為間接螢光染色所偵測，馴化比例僅25％。

關鍵字

傳染性支氣管炎 ； 分離 ； 馴化

並列摘要

Isolation, identification and adaptation of infectious bronchitis virus (IBV) were performed in this study. Twenty-one strains were isolated from 21 outbreak ranches from 1993 to 1995. Most cases occurred in broilers of 4-5 weeks of age and in cold season. Sick chickens always complicated with E. coli as a secondary infection. Thirteen of the occurred ranches had been vaccinated with IBV vaccines. The main pathological lesions included the increase of tracheal secretion and urate deposition in kidney. The isolated viruses were confirmed by electron microscopy as coronaviruses. Adaptation of the 24 isolates (including the former isolates) and 5 reference strains in chicken kidney cell was performed and only 6 of the isolated virus could be adapted in cell (25%). However, all the 5 reference strains could induce cytopathologic effect (CPE) in chicken kidney cell. Only the CPE-induced viruses could be detected by fluorescent staining.

並列關鍵字

Infectious bronchitis ； Isolation ； Adaptation