本省牛群赤羽病抗體分佈與消長情形之調查

The Serological Investigation of Akabane Disease in Taiwan

廖永剛(Yung-Kung Liao) ； 張秋燕(Chiu-Yen Chang) ； 呂榮修(Yung-Siu Lu)

《中華民國獸醫學會雜誌》 22卷2期 (1996/04) Pp. 121-128

https://doi.org/10.7009/JTCSVS.199604.0121

摘要

以本省分離之赤羽病病毒（PT-17株）進行牛群抗體的檢測。檢查本省1989和1990年間發生非化膿性腦膜腦炎病例的牛隻血清，結果證實1989年的181頭血清中有158為抗體陽性（陽性率為89％），1990年之160頭中有149頭為陽性（陽性率為93％）。對1993年間苗栗縣進口牛隻進行赤羽病抗體的追蹤調查發現，1993年4月份進場時陽性率為6％，5月時為24％，6月時31％，至10月時已達96％。顯示牛隻抗體陽性率隨進場時間之增加而上升。再以台南縣4個監測牛群的抗體調查，結果發現抗體的平均力價，由1993年7月至10月間有逐漸升高的趨勢，而在1993年10月時的抗體平均力價最高。而牧場間不同的外圍環境以及氣溫改變也有不同的抗體消長變化。因此比較1994年4月間的抗體力價有陽升現象，並對照牛隻發生類似神經症狀的疾病，顯示本省由春季轉為夏季的這段時間牛群中有赤羽病病毒傳播。1994年間以本省10個縣市1,652頭牛隻，檢測牛赤羽病的中和抗體，結果抗體陰性59頭（3.5％），抗體2倍者37頭（2.2％），抗體力價在4倍或以上者判定為陽性者1,556頭（94.3％）。顯示赤羽病病毒在本省的分佈非常普遍，大部份牛隻均由自然感染而保有赤羽病抗體。

關鍵字

赤羽病 ； 血清學調查 ； 節肢動物媒介病毒

並列摘要

A survey of antibody response in cattle against Akabane virus were conducted. The PT-17 train of Akabane virus isolated from local herd was used as antigen for the serological investigation. 89% (158/181) and 93% (149/160) of the sera from cattle with nonsuppurative encephalitis syndrome showed positive reaction in the antibody test in 1989 and 1990, respectively. The imported cattle in Miaoli district were also monitered the antibody response at monthly intervals in 1993. The positive rate was 6% in April, when the cattle were just transferred into farm, then increased to 24%, 31% and 96% in May, June and October, respectively. The positive rate of imported herd increased as they stayed in farm. Four sentinel cattle herds in Tainan district were tested the antibody distribution every month from July of 1993 to June of 1994. The average antibody titer increased from July to October of 1993, and the highest titer were found in Octorber of 1993. From antibody's response of the sentinel herds in different area, we suspected that the enviroments and climate of the farms would influence the distribution of antibody. The reversion to higher titer were observed in April of 1994, and the same time several abnormal calves were also found in the sentinel herds. The change of antibody responses and abnormal cases in the sentinel herds revealed that Akabane virus was transmitted before April, the time seasonal thift from spring to summer in Taiwan. Furthermore, we collected 1,652 sera of cattle from 10 districts to determine their antibody against Akabane virus in 1994. 94.3% (1556/1652) of the sera had 4 fold or higher titer and recognized to be positive, 2.2% (37/1652) had 2 fold and the rest 3.5% (59/1652) were negative. The high positive rate in antibody response indicated that the cattle in Taiwan had been naturally infected by Akabane virus.

並列關鍵字

Akabane disease ； Arbovirus ； Serological survey