- 期刊
以酵素結合免疫吸附法及間接血球凝集技術測定Eimeria tenella實驗感染雞循環抗體
Enzyme-Linked Immunosorbent Assay and Indirect Haemagglutination Techniques for Measurement of Antibody Responses to Eimeria Tenella in Experimentally Infected Chickens
摘要
試驗目的，係以二種方法來探討Eimeria tenella實驗感染雞之體液性免疫反應，即由芽胞化卵囊獲得純化或半純化的可溶性抗原進行ELISA和IHA二種方法試驗，試驗結果如下：以ELISA和IHA技術測定Eimeria tenella感染雞的抗體，發現EL-ISA 技術比應用IHA較易實施及敏感，且ELISA方法僅需較少量的抗原即可。同樣的血清樣本，ELISA力價最高可達1：65536倍，而IHA為1：128倍；ELISA力價的高低，依據其感染之年齡、接種卵囊之量及接種次數而定。免疫缺陷之雞隻（應用cyclophosphamide處理之雞隻），當其接種數劑量的Eimeria tenella卵囊（分別500，4,500，30,000個），則沒有出現ELISA和IHA之抗體力價。相同年齡雞隻給與同一劑量之卵囊測定其免疫能力，試驗雞隻當其接種Eimeria maxima或Eimeria acervulina而與Eimeria tenella製備之可溶性抗原作用時，以ELISA方法可測到抗體力價為1：50或1：200。而用Eimeria tenella接種雞之血清與相同的抗原作用，則出現較高的抗體力價為1：3200。
並列摘要
The purpose of this study was to compare 2 methods for dectection of the humoral immune response to Eimeria tenella in experimentally infected chickens. A purified or semipurified soluble antigen derived from sporulated oocysts was used in the enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination test (IHA). Results were as follows: An ELISA technigue and IHA test were developed to detect antibodies against Eimeria tenella. The ELISA technigue was relatively easy to perform, more sensitive than the IHA test and needed only a fraction of the antigen reguired for IHA. The highest titers using ELISA were 1:65536 compared to the IHA titers of 1:128 for the same sera. The ELISA titers depened upon the age of the birds when they were infected, the number of oocysts inoculated and the number of inoculations. Immunedeficient birds (cyclophosphamide treated), when inoculated with several dose of oocysts of E. tenella (500, 4500, 30000) showed no ELISA or IHA antibody titers. The immunocompetent chickens of the same age, which received identical doses of oocysts responded with readily detectable antibody levels. Chicken inoculated with E. maxima or E. acervulina had sera titers of 1:50 or 1:200, respectively, when reacted with E. tenella soluble antigen. The E. tenella inoculated birds had titers as high as 1:3200 with the same antigen.
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