Seven flocks of market turkeys were tested for hemagglutination-inhibiting (HI) antibodies against Newcastle disease virus (NDV). Systematic sampling of turkey blood samples (n=305) was done at two turkey packing plants located in the State of Iowa U.S.A. to determine the prevalence of NDV positive reactors. Only one of the seven flocks had been vaccinated against NDV. The geometric mean HI titer (log 2 transformation) for the vaccinated flock was 6.97. Two unvaccinated flocks were seropositive to NDV infections with geometric mean HI titers of 7.78 and 4.95. These 3 NDV positive flocks all had a seroprevalence rate of 100%. The remaining 4 unvaccinated flocks all had HI titers less than 1:8 which was interpreted as NDV free flocks. The implication for human infection from NDV-infected turkeys is stressed.