台灣地區乳牛流產症血清學調查

Serological Survey of Bovine Abortions in Taiwan

鄭江泓(Chiang-Hong Cheng) ； 陳鵬文(Jacky Peng-Wen Chan) ； 馮翰鵬(Hang-Pong Fung)

《中華民國獸醫學會雜誌》 26卷4期 (2000/12) Pp. 293-296

https://doi.org/10.7009/JTCSVS.200012.0293

摘要

為了解台灣地區乳牛流產疫情之發生與Chlamydia psittaci、Neospora caninum、Toxoplasma gondii感染間之關係，由1997年10月至1999年2月間自台灣地區9縣市17個乳牛場，進行檢體採集及流產疫情調查，共計採得390例牛隻血清及29例牧場犬隻血清，並由中興大學獸醫教學醫院分讓家犬血清26例。採得之牛隻血清進行C. psittaci、N. caninum及T. gondii抗體檢測，而犬隻血清則檢測其N. caninum、T. gondii抗體。結果在390例牛隻血清中，C. psittaci抗體陽性率為29.2% (114/390), N. caninum抗體陽性率為33.1% (129/390)而T. gondii抗體陽性率為1.1% (4/370)。在血清學與流產症關係方面，C. psittaci抗體陽性率在12個流產牧場(29.0%)與5個對照牧場(29.7%)及63頭流產牛(33.3%)與164頭對照牛(31.7%)之間並無顯著差異(P＞0.05)。N. caninum抗體陽性率在流產牧場(46.4%)與對照牧場(8.7%)及流產牛(68.3%)與對照牛(15.2%)間皆呈現極顯著差異(P＜0.01)。犬隻血清檢驗結果在29例牧場犬血清中，N. caninum抗體陽性率為58.6% (17/29), T. gondii抗體陽性率為84.0% (21/25)，家犬血清N. caninum抗體陽性率為30.8% (8/26), T. gondii抗體陽性率為34.6% (9/26)，牧場犬隻與家犬N. caninum抗體陽性率間呈現顯著差異(P＜0.05)，而牧場犬隻與家犬T. gondii抗體陽性率間則呈現極顯著差異(P＜0.01)。由血清學調查結果顯示，本省乳牛感染C. psittaci及N. caninum的情形相當普遍，其中以N. caninum與流產症間有極顯著的相關性，為今後流產診斷不可忽視之病原。

關鍵字

牛流產症 ； 披衣菌 ； 新孢蟲 ； 弓蟲

並列摘要

This study was aimed to investigate the infection situation of various cattle abortion relevant pathogens such as Chlamydia, Neospora, and Toxoplasma in Taiwan. Within the period of October 1997 and February 1999, a total number of 390 bovine serum specimen and 29 canine serum specimen were collected from 17 dairy farms located in 9 different counties. Besides, 26 canine specimen were obtained from the veterinary medical teaching hospital of Chung-Hsing University. The presence of serum antibody against C. psittaci was detected by use of the commercial CHEKIT^® EIA kit while N. caninum, and T. gondii by IFA methods. Cattle serum was examined for C. psittaci N. caninum and T. gondii while canine serum for the latter two. The cattle seroprevalence of antibody against C. psittaci N. caninum and T. gondii were 29.2% (114/390), 33.1% (129/390) and 1.1% (4/370), respectively. There was no significant difference (P＞0.05) between aborted cows and those in control herds in terms of association of presence of antibody against C. psittaci and abortion. Similar results were also seen in abortion herds and those herds served as control. However, in respect to N. caninum the seroprevalence of antibody in aborted cows was different from non-aborted cows (P＜0.01). And, antibody present in abortion herds was similar to control herds (P＜0.01). Moreover, it appeared to be an association between presence of antibody against N. caninum and incidence of abortion in cows. The seroprevalence of antibodies against N. caninum in dogs resident around abortion herds (58.6%) were higher than those from teaching hospital (30.8%). In respect to T. gondii the detectable antibody was 34.6% in all dogs investigated while significant difference was found between two dog sources (P＜0.01). The result of present serological survey revealed that the existing of infections of C. psittaci and N. caninum in cattle in Taiwan. The striking association between N. caninum infection and incidence of bovine abortion, ought to be noticed for the better understanding of cow abort ion of obscure causes.

並列關鍵字

Bovine abortions ； Chlamydia psittaci ； Neospora caninum ； Toxoplasma gondii