Detection of Pseudorabies Virus in Tissues Using In Situ Hybridization and Polymerase Chain Reaction Techniques

以假性狂犬病病毒（PT株）接種豬隻三個月後採集各組織臟器，並以福馬林固定，石蠟包埋組織，以毛地黃素標示核酸當探針，再以原位雜合及原住聚合酶連鎖反應偵測組織內潛伏感染之假性狂犬病病毒。為了進一步確認原位雜合及原位聚合酶連鎖反應之特異性，以聚合酶連鎖反應增幅本病毒既早期(IE)和醣蛋白E(gE)基因。從結果得知以原位檢測方法可以快速和正確的診斷本病毒的潛伏性感染。比較這兩種技術的敏感性，原位聚合酶連鎖反應比原住雜合反應更敏感。故以原位聚合酶連鎖反應是一種快速、敏感性和特異性高的技術可偵測潛伏感染之假性狂犬病病毒。

關鍵字

假性狂犬病病毒；原位雜合反應；原位聚合酶連鎖反應

並列摘要

An in situ hybridization (ISH) and in situ polymerase chain reaction (in situ PCR) tests with a digoxigenin (DIG)-labeled DNA probe were used to detect the latency of pseudorabies virus (PRV) DNA in formalin-fixed, paraffin-embedded pig tissues. Tissues were collected 3 months following inoculation with the PT strain of PRV. To confirm the specificity of ISH and in situ PCR. PCR was used to amplify the IE and gE genes of the PRV PT strain. In situ methods are more rapid and accurate for the diagnosis of latent PRV infections. The sensitivity of these two techniques was compared. The in situ PCR test was found to be more sensitive than ISH and allowed the rapid, sensitive, and specific detection of latent PRV infections.

並列關鍵字

Pseudorabies virus ； in situ hybridization ； in situ PCR

國際替代計量

Detection of Pseudorabies Virus in Tissues Using In Situ Hybridization and Polymerase Chain Reaction Techniques

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