應用高效液相層析法作甘草及其製劑中Glycyrrhizin之鑑別及含量測定

High Performance Liquid Chromatographic Analysis of Glycyrrhizin in Licorice & It's Pharmaceutical Preparations

陳婉淑(Wan-Su Chen) ； 秦玲(Ling Chyn) ； 許秀錦(Show-Jiin Sheu) ； 陳本(Been Chen) ； 鄭建詒(Chien-Yi Cheng)

《藥物食品檢驗局調查研究年報》 3號 (1985/09) Pp. 59-63

https://doi.org/10.6946/ASRNL.198509.0059

摘要

以2N鹽酸溶液在沸騰水浴上加熱90分鐘將甘草主成分甘草甜素(Glycyrrhizin)水解為一分子甘草次酸(Glycyrrhetinic acid)及二分子葡萄糖醛酸(Glucuronic acid)後，應用高效液相層析法(High Performance Liquid Chromatogrphy；HPLC)分離並定量甘草次酸；本實驗法可供甘草藥材及其製劑中Glycyrrhizin之鑑別及含量測定，其分離效果良好，精確度高，敏感度為0.1μg。以此法測定17種甘草藥材中Glycyrrhizin含量範圍在3.0~9.2%。

並列摘要

Glycyrrhizin in Licorice and it's pharmaceutical preparations is decomposed to one molecular Glycyrrhetinic acid and two molecular Glucuronic acid by 2N Hydrochloric acid, then we apply a reverse phase High Performance Liquid Chromatographic method to analysis Glycyrrhetinic acid so as to identify and determine the quantity of Glycyrrhizin indirectly. This method results great separation, accuracy and high reproducibility could be applied to routine analysis of Glycyrrhizin.