In this experiment we developed three types of mobile phase systems in TLC to identify Bupleuri Radix and its Chinese preparations. The mobile phase systems consist of: Ethylacetate-Methanol-Water (10:2:1), n-Butanol-Glacial Acetic Acid-Water (4:1:2), Chloro form. In these TLC mobile phase systems, We can easily separate Bupleuri Radix in Long Dan Shieh Gan Tong(龍膽瀉肝湯), Jia Wey Shiau Yau Sann (加味逍遙散), Buu Jong Yih Chih Tong(補中益氣湯), Conquently, we clearly identified the spots showing Bupleuri Radix by using P-Dimethlyamino benzaldehyde spray reagent or U. V. light (366nm).