Morphogenesis, Biomass and Oxytetracycline Production of Streptomyces rimosus in Submerged Cultivation

Streptomyces rimosus在浸沉培養時之形態化育、生質量與地靈黴素生產

王貞懿(Jen-Yih Wang) ； 楊盛行(Shang-Shyng Yang)

《藥物食品檢驗局調查研究年報》 14號 (1996/09) Pp. 68-78

https://doi.org/10.6946/ASRNL.199609.0068

摘要

以分光光度儀、HPLC和螢光素－螢光素酶法測定ATP時，其最低之檢出濃度分別為1.0μM，3.3μM和100nM。在浸沉培養65小時時，大部分之Streptomyces rimosus TM-55以菌絲片斷存在，培養168小時則形成短桿狀菌絲，而培養504小時則有部分菌絲被分解。培養24小時時其ATP含量最高，而後隨培養過程而逐漸降低。地靈黴素產量亦隨培養過程而增加，於166小時達最高力價178.9μg/ml而後逐漸下降。形態化育在Streptomyces浸沉培養生產地靈黴素時佔很重要之角色，短菌絲片段較有利於抗生素生產。

並列摘要

The minimal concentration of adenosine triphosphate (ATP) which could be detected with spectrophotometry, HPLC and luciferin-luciferase methods was 1.0μM, 3.3μM and 100nM, respectively. In submerged cultivation, most Streptomyces rimosus TM-55 was in hyphae fragment form at 65 h, became short-rod mycelia at 166 h, and lysed at 504 h incubation. The ATP content had maximal value at 24 h, then gradually decreased during cultivation. The oxytetracycline potency increased as incubation occurred, had maximal potency 178.9 μg/ml at 166 h, and then gradually decreased. Morphogenesis was very important in oxytetracycline production in submerged cultivation of Streptomyces; short-rod mycelia had high oxytetracycline production.

並列關鍵字

Morphogenesis ； Streptomyces rimosus ； oxytetracycline potency ； ATP content ； submerged cultivation