- 期刊
利用高效液相層析儀鑑別含細辛中藥製劑之方法研究
High Performance Liquid Chromatography Identification Study on Aristolochic Acid in Traditional Chinese Medicinal Prescriptions Containing Asiasarum Heterotropoides
摘要
本計畫選擇小青龍湯等八種含細辛之中藥製劑，建立其高效液相層析法，以用於市售製劑中馬兜鈴酸之鑑別。經實驗結果顯示，八種處方使用C-18之層析管，以0.05mol/L NaH2PO4(H3PO4 2mL): CH3CN=65: 35及0.05mol/LNaH2PO4(H3PO42mL): MeOH: CH3CN=55:10:35二種移動相，其分離效果良好。檢量線之相關係數均為0.999以上，而aristolochic acid I在儀器之最低檢出量及製劑中之最低檢出量分別為3.742ng及3.792ng，八個處方之添加回收率則介於95.5%~107.8%之間。
並列摘要
A high performance liquid chromatography (HPLC) method for the identification of aristolochic acid in traditional Chinese medicinal prescritions containing Asiasarum heterotropoides was established. The prescription included Sheau-Qing-Long-Tang (小青龍湯)、Chuan-Chyong-Char-Tyau-Saan (川芎茶調散)、Dwu-Hwo-Jih-Sheng-Tang (獨活寄生湯)、Jeou-Wey-Chiang-Hwo-Tang (九味羌活湯)、Ma-Hwang-Fuh-Tzyy-Shih-Shin-Tang (麻黃附子細辛湯)、Ching-Shang-Jiuan-Tong-Tang (清上蠲痛湯)、Dah-Chyn-Chyou-Tang (大秦艽湯) and Yeh-Gan-Ma-Hwang-Tang (射干麻黃湯).The samples were analyzed using the HPLC method on a Ace 3C18 column and were detected at 400nm with 0.05mol/L Sodium Biphosphate(2mL Phosphoric acid) and acetonitrile(65:35), 0.05mol/L Sodium Biphosphate (2mL Phosphoric acid) and methanol and acetonitrile (55:10:35) as the mobile phase at a flow rate of 1.0ml/min。The linear coefficient of regression equation of all weremore than 0.999, the detection limit of Aristolochic acid I for instrument and samples were 3.742 ng and 3.792 ng respectively，the recoveries of eight samples were 95.5%~07.8%.
延伸閱讀
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