應用二種管柱後衍生化反應分析食品中黃麴毒素之比較

Comparison of Two High-Performance Liquid Chromatography with Post-Column Derivatization Systems for Testing Aflatoxins in Food

陳曉錚(Hsiao-Cheng Chen) ； 李衛宗(Wei-Chung Li) ； 談國雄(Gwo-Shyong Tan) ； 闕麗卿(Lih-Ching Chiueh) ； 傳幼敏(You-Min Fu) ； 施養志(Daniel Yang-Chih Shih)

《藥物食品檢驗局調查研究年報》 24號 (2006/12) Pp. 421-430

https://doi.org/10.6946/ASRNL.200612.0421

摘要

為建立更有效能之食品中黃麴毒素檢驗方法，以高效液相層析管柱後光化學反應測試黃麴毒素B1、B2、G1及G2，與衛生署公告指定方法之高效液相層析管柱後碘衍生化反應比較，結果顯示二種反應所得之標準曲線線性及方法偵測靈敏度皆佳且相似；另就其他效益比較，光化學反應具有操作簡易、分析所需時間較短及設備費用較低等優點。

關鍵字

高效液相層析 ； 管柱後衍生化反應 ； 黃麴毒素

並列摘要

In order to establish more efficiency aflatoxin test method in food, high performance liquid chromatography is used with post-column in-line photochemical derivatization (PCD) to determinate level of aflatoxin B1, B2, G1 and G2 in food. Comparing with the official method announced by Department of Health of Taiwan using high-performance liquid chromatography with post-column derivatization by I2 (I2D), the results showed that linearity of standard curves and sensitivity of detection obtained by PCD and I2D were equivalent. However the operating procedure, analytical time and cost of apparatus of PCD are easier, shorter and lower than I2D, respectively.

並列關鍵字

high performance liquid chromatography ； post-column derivatization ； aflatoxin