利用植物抗病活性劑25ppm BTH(benzo(l, 2, 3)thiadiazole-7-carbothioic acid S-methyl ester),在台北市台大水源校區進行四個月的月橘白粉病(由Oidium murrayae引起)的防治試驗。2002年12月修剪月橘圍籬過後,白粉病尚未發生時,即開始進行月橘白粉病的防治試驗。藥劑處理過的圍籬試區,每個月調查一次,連續4個月的觀察,發現BTH具有顯著減輕與遲緩白粉病發生的效果。施藥後3個月期間,處理BTH試區之植株在各月的平均受感染率分別為4%、15%及32%,而對照圍籬試區則為9%、42%及61%。2003年2、3及4月連續調查資料,經Logistic regression統計分析,顯示施用藥劑對白粉病的發病率有顯著的影響,有BTH處理之試區,其2、3、4月及三個月綜合之白粉病感染率分別為對照組之0.67、0.43、0.47及0.55倍,顯示有藥劑處理者白粉病感染率較低。在實驗室中以1000、100、25、10ppm之BTH處理白粉病孢子,發現BTH對白粉病孢子的發芽率高低並無影響。本研究發現,25ppm的BTH雖然無法殺死白粉病孢子或抑制孢子發芽,但可降低月橘白粉病的感染率,其作用可能與誘導抗病有關,但需進一步之研究。
Common jasmin orange seriously infected by powder mildew (caused by Oidium murrayae cf. Hosag., U. Braun & Babindran) during 1999-2001, in National Taiwan University Shuiyuan District were chosen to perform the powdery mildew control experiment for four months. The main purpose of this study was to evaluate the effect of BTH (benzo (l, 2, 3) thiadiazole-7-carbothioic acid S-methyl ester) at 25 ppm on control of powdery mildew of the common jasmin orange. The experiments with a one-way completely randomized design were conducted in January 2003. Disease severity was investigated once per month from February to April 2003. The average disease severity were 40%, 15%, and 32% respectively for the treated areas, and 90%, 42% and 61% for the control ones. The data were analyzed by Logistic regression. The result indicated that BTH at 25 ppm was significantly effective in reducing the infection by the pathogen compared to control plots. The odds ratios were 0.67, 0.43, 0.47 times for each month, and in total 0.55 times. The spores of powdery mildew fungus were treated with 1000, 100, 50, 25, 10 ppm of BTH separately in 2% water agar. Results showed that BTH was not able to inhibit spore germination at 25 ppm. However, it could significantly reduce the disease severity. The study sugguests that BTH might induce common jasmine orange plant to resist powdery mildew.