以傅立葉轉換紅外線光譜與近紅外線光譜解析黴菌對纖維素化學結構的降解作用

Illustrating the Deterioration of Chemical Structure of Mildew-Damaged Cellulose by Fourier Transform Infrared Spectroscopy (FTIR) and Near Infrared Spectroscopy (NIR)

楊菀仁(Wan-Jen Yang) ； 張上鎮(Shang-Tzen Chang) ； 宋子萱(Tzu-Hsuan Sung) ； 張惠婷(Hui-Ting Chang)

《國立臺灣大學生物資源暨農學院實驗林研究報告》 34卷4期 (2020/12) Pp. 365-374

https://doi.org/10.6542/EFNTU.202012_34(4).0006

摘要

本研究以傅立葉轉換紅外線光譜與近紅外線光譜分析經黴菌Aspergillus niger與Chaetomium globosum危害後的濾紙，瞭解黴菌對濾紙纖維素化學結構的影響。由黴菌危害濾紙的傅立葉轉換紅外線光譜中，觀察到真菌細胞壁成分官能基Amide I與Amide II的紅外線吸收峰，迅號隨試驗時間而增強，纖維素特性吸收峰的強度逐漸減弱，顯示纖維素受黴菌酵素降解而使化學結構破壞。使用近紅外線光譜分析黴害濾紙，同樣可觀測到因黴菌附著，形成新的近紅外線吸收峰，而濾紙纖維素的特性吸收峰強度降低。

關鍵字

生物劣化 ； 纖維素 ； 傅立葉轉換紅外線光譜 ； 黴菌 ； 近紅外線光譜

並列摘要

In this study, we analyzed the deterioration of chemical structure of filter paper caused by mildews including Aspergillus niger and Chaetomium globosum by Fourier transform infrared spectroscopy (FTIR) and near infrared spectroscopy (NIR). Results from FTIR spectra of mildew-damaged filter papers showed that new signals related to Amide I and Amide II groups of fungal cell wall were detected and increased with incubation time. The intensities of characteristic bands of cellulose were decreased, which revealed that the chemical structure of cellulose was deteriorated by enzymes secreted from mildew. New characteristic peak of mildew was also observed in NIR spectra of mildew-damaged filter papers; the absorption bands of cellulose were consistently decreased after the mildew test.

並列關鍵字

Biodegradation ； Cellulose ； Fourier transform infrared spectroscopy ； Mildew ； Near infrared spectroscopy