A new technique for storage of meristem-tip cultures of Cavendish' banana

香蕉組織培養長期保存方法

Wen-Hsiung Ko ； Shin-Chuan Hwang ； Fan-Mei Ku

《台灣香蕉研究所年報》 1990年 (1991/05) Pp. 69-74

摘要

Meristem-tip tissues of Cavendish' banana were incubated at 17°C under light on cheesecloth over cotton saturated with 3% solution of various kinds of sugars. All the sugars tested were found to be capable of extending the survival time of banana tissues and ribose was the best followed by sucrose, glucose, fructose and lactose. After 21 months, 33% of banana tissues incubated with ribose solution remained viable and resumed growth within two weeks after being transferred to fresh SM emdium. About 99% of plantlets devloped from these tissues were normal. In a separate experiment, 67% of banana tissues incubated with 3% ribose solution remained viable after 24 months. Ribose solutions at 0.3% and 0.03% were not as effective and over saturation of cotton with 3% ribose solution was not beneficial. The new technique may be useful for storage of tissue cultures of other species and cultivars.