This work aimed to utilize an Oriel spectrograph named MS260iTM with silicon photodiode array spectrophotometer in the wavelength range between 900 and 1100 nm for on-line pear fruits grading. Spectra of forty-four pear fruits scanned on-line, pretreated by second derivative method, and then analyzed by PLSR model supplied by Unscrambler ® software. The calibration curve of sugar content in pear fruits had correlation coefficient and standard error of cross validation 0.698, 0.335 °Brix, respectively. The result shows the possibility of using NIR spectroscopy in the pear grading process in the future provided that the RPD value will be increased by broadening the standard deviation of sugar content in pear samples and maintained the SECV value and appropriate sorting instruments are developed.