This study was intended to explore the effects of light quality on the seedling quality for bitter gourds at a controlled environment room. Experimental results showed the seedling quality of RGB (51:24:25) treatment was the best at 150 μmol m^(-2) s^(-1) light intensity, and plant height, stem diameter, number of leaf, root length, fresh weight of shoot, fresh weight of root, dry weight of shoot, dry weight of root, dry weight of plant, seedling index were 23.42 cm、3.07 mm、8.83 leaves、29.75 cm、4.51g、3.82 g、0.85 g、 0.29 g、1.14 g and 1.34, respectively. This study confirmed the environmental control room by using the artificial light source to replace the natural sunlight for bitter gourds seedling production was feasible.