- 期刊
- OpenAccess
以稉稻重組自交系族群定位劍葉形態之數量性狀基因座
QTL Mapping for Flag Leaf Characters by Japonica Rice Recombinant Inbred Lines
摘要
本試驗為瞭解稉稻劍葉的控制基因位置與其遺傳效應，以144 個稉稻（越光/臺農67 號）重組自交系為材料調查劍葉長、寬及面積，另於生育適期評估抽穗期、株高及分蘗數等性狀。結合115 個SSR 分子標誌的分析結果，共可定位測得10 個數量性狀基因座（QTL）。影響劍葉長表現之qFLL-3 及qFLL-10，其概度對數值（LOD）為3.4 及4.7，對試驗族群的外表型變異解釋量（PVE）各為8.6%及15.6%，以qFLL-10的效應較大；控制劍葉寬表現的2 個QTL（qFLW-3 及qFLW-8），LOD 值為5.3 及3.4，對劍葉寬度的變異解釋率為24.5%及11.1%，以qFLW-3 的表現效應較為顯著；而與劍葉面積表現相關者僅可測得1 個QTL（qFLA-10），LOD 及PVE 各為5.9 及18.3%。其他關於抽穗期及株高表現，經分析後可測得3 個（qHD-6、qHD-8 及qHD-10）及2 個QTL（qPH-3 及qPH-10）。此外，本試驗又調查各行系的稻穀產量，分析其與劍葉性狀的相關性，結果顯示劍葉寬及劍葉面積與產量呈顯著正相關。因此，如欲提高光合作用供源，改善劍葉長、寬及面積等性狀，可以第3 條染色體上qFLW-3 附近的RM6736，與位於第10 條染色體上qFLL-10 及qFLA-10 附近的RM5304~RM6704區間，作為輔助選拔使用。
並列摘要
To understand the locations and genetic control effects about flag leaf in rice, 144 recombinant inbred lines derived from japonica rice cross (Koshihikari / Tainung 67) were used to measure flag length, flag width and flag area. Heading date, plant height and tiller numbers of each lines were also investigated in proper growth stage. Combined with 115 SSR markers, totally 10 quantitative trait loci (QTL) were mapped. The likelihood ratio of odds (LOD) of two QTL (qFLL-3 and qFLL-10) which affected flag leaf length were 3.4 and 4.7, respectively. The phenotypic variance explained (PVE) were 8.2% and 15.6% corresponding to qFLL-3 and qFLA-10, respectively. qFLL-10 had the greatest influence. The LOD and PVE among two QTL(qFLW-3 and qFLW-8)which controlled flag leaf width were 5.3 / 3.4 and 24.5% / 11.1%, respectively. qFLW-3 showed the most significant effect. Only one QTL were detected to associate with flag leaf area, which LOD / PVE value was 5.9 / 18.3% corresponding qFLA-10. This trial had also detected three (qHD-6, qHD-8 and qHD-10) and two (qPH-3 and qPH-10) QTL which responded for controlling heading date and plant height. In addition, grain yield of each line was also investigated and it showed significantly positive correlation with flag leaf width and flag leaf area. In order to increase photosynthesis source by improving flag leaf length, flag leaf width and flag leaf area, RM6736 nearby qFLW-3 on chromosomes No. 3 and interval between RM5304 and RM6704 nearby qFLL-10 and qFLA-10 on chromosomes No.10, could be further utilized for selection.