檢體之貯存溫度與時間對臨床檢驗結果之影響

Influence of Sample Storage on Clinical Chemistry Tests

蔡麗玉(Li-Yu Tsai) ； 江季勛(Chi-Hsiun Chiang)

《The Kaohsiung Journal of Medical Sciences》 1卷12期 (1985/12) Pp. 722-730

https://doi.org/10.6452/KJMS.198512.0722

摘要

To understand the influence of sample storage on routine clinical chemistry tests, we collected 12 specimens of serum from normal subjects, which were stored at different temperatures for different lengths of period to measure the stability of various clinical chemistry tests.
The routine clinical chemistry tests included total protein, albumin, asparate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, urea nitrogen, creatinine, high density lipoprotein cholesterol, total cholesterol, and triglyceride. The statistical significance was computed on the basis of Wilcoxon’s signed ranks test.
The stability of various constituents in human serum was investigated after storage at room temperature, 4°C and —20°C under various storage duration.
The results were summarized as follows:
1. At room temperature, serum protein was stable for 2 days while albumin was stable for 5 days. All enzymes except lactate
dehydrogenase were stable for 5 days, and urea nitrogen, creatinine, high density lipoprotein cholesterol, and triglyceride were stable for 2 days. Total cholesterol was stable for 5 days.
2. At 4°C, serum protein and albumin were stable for 5 days, while asparate aminotransferase and alkaline phosphatase were stable for 2 days. The other enzymes were stable at least for 5 days. Urea nitrogen and creatinine were stable for 6 days and 2 days respectively, and total cholesterol and triglyceride were more stable than high density lipoprotein cholesterol.
3. At —20°C, serum protein and albumin were stable for 8 days. All enzymes except lactate dehydrogenase were stable for 8 days at least. Urea nitrogen and creatinine were stable for 40 days and 8 days respectively, and lipids were stable for 40 days at least.
4. All constituents in human serum except alkaline phosphatase, total cholesterol and triglyceride decreased in concentration after storage.

並列摘要

To understand the influence of sample storage on routine clinical chemistry tests, we collected 12 specimens of serum from normal subjects, which were stored at different temperatures for different lengths of period to measure the stability of various clinical chemistry tests.
The routine clinical chemistry tests included total protein, albumin, asparate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, urea nitrogen, creatinine, high density lipoprotein cholesterol, total cholesterol, and triglyceride. The statistical significance was computed on the basis of Wilcoxon’s signed ranks test.
The stability of various constituents in human serum was investigated after storage at room temperature, 4°C and —20°C under various storage duration.
The results were summarized as follows:
1. At room temperature, serum protein was stable for 2 days while albumin was stable for 5 days. All enzymes except lactate
dehydrogenase were stable for 5 days, and urea nitrogen, creatinine, high density lipoprotein cholesterol, and triglyceride were stable for 2 days. Total cholesterol was stable for 5 days.
2. At 4°C, serum protein and albumin were stable for 5 days, while asparate aminotransferase and alkaline phosphatase were stable for 2 days. The other enzymes were stable at least for 5 days. Urea nitrogen and creatinine were stable for 6 days and 2 days respectively, and total cholesterol and triglyceride were more stable than high density lipoprotein cholesterol.
3. At —20°C, serum protein and albumin were stable for 8 days. All enzymes except lactate dehydrogenase were stable for 8 days at least. Urea nitrogen and creatinine were stable for 40 days and 8 days respectively, and lipids were stable for 40 days at least.
4. All constituents in human serum except alkaline phosphatase, total cholesterol and triglyceride decreased in concentration after storage.

並列關鍵字

ample storage ； clinical chemistry tests