The Effect of Protamine Sulfate on Gastric Acid Secretion and Histamine Release

魚精蛋白對胃酸分泌的作用與組織胺釋出之關連研究

陳英俊(Ing-Jun Chen) ； 呂榮銘(Rong-Ming Lyu)

《The Kaohsiung Journal of Medical Sciences》 2卷5期 (1986/05) Pp. 310-319

https://doi.org/10.6452/KJMS.198605.0310

摘要

測定大白鼠注射protamine sulfate 所導致的胃酸分泌增加量，發現反應增加量與注射劑量有相關性。同一隻大白鼠經注射兩protamine sulfate (20 mg/kg) 可導致幾乎相同的胃酸分泌增加量；故此種注射劑量尚不致於引起胃酸分泌之快速防禦反應(tachyphylaxis)
分析魚精蛋白注射(20mg/kg)所導致的胃酸分泌，發現2/3的大白鼠呈中等程度的酸分泌反應(2.5～8.5μEq)，而少數個體產生較強反應(12.5μEq)。受檢的65隻大白鼠之胃酸分泌反應呈對數常態分佈。若由靜脈注射protamine sulfate前30分鐘先由腹腔各別注射cimetidine（2 mg/kg），或cromolyn sodium（10mg/kg）或atropine sulfate（5mg/kg）則可阻止protamine sulfate所引起之胃酸分泌。
測注射前後，血漿中histamine含量的變化，可發現protamine sulfate（30mg/kg）有意地增加血漿中histamine含量。
以pH-stat的方法對牛蛙胃黏膜離體組織測定胃分泌；此種刺激胃酸分泌的作用，對經過cimetidine（10-4g/ml）前處理的胃黏膜亦有同樣的效果；由漿膜面淬取液發現protamine sulfate使組織胺之釋出增加。
綜合120隻大白鼠及22個牛蛙胃黏膜離體組織的試驗，protamine sulfate確定可引起胃酸分泌。此種現象與組織胺的釋出有關。因為經由組織胺來促使胃酸分泌增加所須的histamine量很大，故protamine sulfate注射引起的其它副作用，也可能以此種產生組織胺的釋出為最重要的因素。

並列摘要

１. Gastric acid secretion (GAS) of rats was dose-dependently increased by intravenous injection of 10-40 mg/kg protamine sulfate (PS). The frequency of occurrence vs. total acid output induced by each injection of PS (20 mg/kg) reavealed a statistical lognormal distribution in 65 rats.
２. Treatment with pyrilamine (5mg/kg, i.p.) or diphenhydramine (5 mg/kg, i.p.) 30 min before the injection of PS failed to inhibit the protamineinduced gastric secretion (PIGAS). However, atropine (5 mg/kg), cromolyn sodium (10mg/kg, i.p.) of cimetidine (2 mg/kg, i.p.) inhibited this effect.
３. Acid secretion and histamine-release from isolated bullfrog gastric mucosal preparations were measured in vitro by a recording pH stat and fluorometric asay. PS (2×10-4g/ml)increased GAS and histamine-release in a mucosal solution and serosal solution respectively. After the spontaneous secretion was decreased by treatment with cimetidine (1×10-4g/ml)and washed with serosal solution. Further application of PS restored acid secretion and histamine-release.
４. It is concluded that PS induces histamine-release (both in vivo and in vitro) from histamine-containing cells, basophil leucocytes and mast cells, and thereafter stimulates the acid secretion from parietal cells.

並列關鍵字

protamine sulfate ； gastric acid secretion ； histamine release