口腔粘膜下纖維性病變其膠原蛋白含量與類型之研究

Collagen Content and Types in Oral Submucous Fibrosis

黃逸岳(I-Yueh Huang) ； 謝天渝(Tien-Yu Shieh)

《The Kaohsiung Journal of Medical Sciences》 5卷3期 (1989/03) Pp. 162-171

https://doi.org/10.6452/KJMS.198903.0162

摘要

為探討口腔粘膜下纖維性病變其膠原蛋白含量與類型的變化，本研究將病患口腔粘膜組織及正常之口腔粘膜和皮膚組織，以胃蛋白酶和磷酸氫鈉萃取出膠原蛋白，分析其胺基酸組成與膠原蛋白含量，並以電泳分析法，比較其類型變化。結果發現正常口腔粘膜其膠原蛋白的特性，如含量、類型及類型比例等與正常皮膚者，有些微差異，但均無統計學上差異。口腔粘膜下纖維性病變其膠原蛋白含量與組織學上顯示的膠原纖維堆積程度一致，重度病變組 (221.6±58.2μg/ml)比中度病變組 (107.1±37.8μg/ml) 和正常口腔粘膜 (111.8±31μg/ml) 均高，且有統計學上差異 (p＜0.001)。在膠原蛋白胺基酸組成方面，口腔粘膜下纖維性病變與正常口腔粘膜相似，唯Hydroxyproline、proline、Glycine和Alanine等略為偏低，另由Hydroxyproline推算膠原蛋白總含量所引用之轉換係數，本研究所得結果，正常皮膚為9.21，正常口腔粘膜為8.52，口腔粘膜下纖維性病變組織為10.15。在膠原蛋白類型和類型比例方面，正常口腔粘膜和纖維性病變組織均呈現類型Ⅰ、Ⅲ、Ⅴ三種類型，Ⅲ／Ⅰ比值：正常口腔粘膜為0.119±0.030，口腔粘膜下纖維性病變中度病變組為0.142±0.059，重度病變組為0.118±0.074：Ⅴ／Ⅰ比值：正常口腔粘膜為0.024±0.011，中度病變組為0.032±0.01，重度病變組為0.049±0.023，兩者在統計學上無差異，但Ⅴ／Ⅰ比值，有隨疾病之進行而增加的趨勢。

並列摘要

Oral submucous fibrosis is a chronic disease of the oral cavity. The basic histological change which occurs is a fibroelastic transformation of the connective tissue in the laminal propria layer associated with epitherial atrophy. The etiology of the disease is uncertain, but there is a close association suggested both geographically and epidemiologically with the habitual chewing of betelnuts.
The accumulation of collagen fibers increases with the severity of the disease, and the fibroblasts in the normal mucosa and in the fibrotic mucosa increased their proliferation and collagen synthesis. This can be activated by arecoline, an extract from betel nuts, as described in a recent study. In order to obtain some information about the basic characteristics of the collagen in submucous fibrosis and its correlation with the fibrotic changes, the following study wad condueted.
In this study, collagen was extracted from the tissues of normal mucosa, normal skin and oral submucous fibrosis with pepsin and disodium hydroxyphosphate. The amino acid compositions of collagen, collagen content, types and their ratios were measured and analyzed. The results indicated that the characteristics of collagen in normal mucosa and skin were similar in content (normal mucosa: 111.8＋31μg/ml; normal skin:131.4＋56.4μg/ml), amino acid compositions, types (Ⅰ,Ⅲ,Ⅴ), and ratios of different types (Ⅲ/Ⅰ: normal mucosa: 0.119＋0.03; normal skin: 0.187＋0.046, Ⅴ/Ⅰ: 0.024＋0.01; 0.0036＋0.01). Collagen content in the advanced group with oral submucous fibrosis (221.6＋58.2μg/ml) was higher than that of the normal mucosa group (111.8＋31μg/ml) and the moderate group with oral submucous fibrosis (107.1＋37.8μg/ml) by a significant difference. Put no difference occurred between normal mucosa and moderate group with oral submucous fibrosis. The collagen of normal skin, normal mucosa and oral submucous fibrosis (both the advanced &moderate groups) had similar amino acid compositions, except that the presence of hydroxyproline, praline, and glycine were less in oral submucous fibrosis. The conversion factors for determining the total collagen, done by measuring the concentration of hydroxyproline, were 10.15 for oral submucous fibrosis, 9.21 for normal skin, and 8.52 for normal mucosa. Normal skin, normal mucosa, and oral submucous fibrosis have the same collagen types (Ⅰ,Ⅲ,Ⅴ). The ratios of type Ⅲ to type Ⅰcollagen and type Ⅴ to type Ⅰwere compared between every two groups and the result showed no significant difference.
In this study, the basic characteristics of collagen in oral submucous fibrosis were investigated. Further studies on oral submucous fibrosis regarding etiology, diagnosis, and treatment, are yet needed.

並列關鍵字

oral submucous fibrosis ； collagen content ； collagen types