金黃色葡萄球菌之抗性及其質體之探討

本試驗自高雄醫學院中和紀念醫院計收集金黃色葡萄球菌62株，經抗生素及重金屬離子之感受性試驗結果，顯示所收集菌株對多種抗菌劑具有抗藥性，其中對methicillin之抗性高達33.9%，而gentamicin為9.7%，至於重金屬離子之抗性以As (95%)及Cd (76%)偏高。此外多重抗性普遍存在，以nalidixic acid-tetracycline, ampicillin-nalidixic acid-tetracycline及 ampicillin-chloramphenicol-kanamycin-streptomycin-tetracyclin等較為常見。噬菌體分型以groupⅠ居多占38.7%，尤以52型多達27%，其次為groupⅠ&Ⅲ混合型 (20.9%)，其中以52／83A型居優勢 (11%)。此等型別之菌株與ampicillin, chloramphenicol, streptomycin, nalidixic acid, kanamycin及methicillin等藥物之抗性富密切關係。經DNA分析結果發現金黃色葡萄球菌普遍存有分子量互異之質體，且由質體之去除與抗性關係顯示27×106 dal質體為攜帶ampicillin及kanamycin抗性基因，而30×106 dal質體之存在則使菌株對ampicillin, kanamycin及streptomycin呈抗性，3×106 dal質體為攜帶chloramphenicol之抗性基因，至於tetrcycline之抗性基因則存於2.8×106 或28×106 dal質體上。利用HindⅢ限制酶切取以比較質體DNA顯示抗性相同而分子量互異之質體彼此間富相似性。

關鍵字

無資料

並列摘要

A total of 62 clinical strains of Staphylococcus aureus collected from Kaohsiung Medical College were examined for susceptibility of several antimicrobial agents and underwent plasmid analysis. The results of the antibiotic susceptibility test revealed that 33.9% were resistant to methicillin and 9.7% were resistant to gentamicin, whereas, resistance to heavy metal ions such as arsenic and cadmium were as high as 95% and 76% respectively. Antibiotic multiresistant isolates of S. aureus were also found. Multiresistance to nalidixic acid-tetracycline, ampicillin-nalidixic acid-tetracycline, and ampicillin-chloramphenicol-kanamycin-streptomycin-tetracycline weremost frequently encountered. The phage typing results indicated that type 52 was predominant in 27%, followed by 11% of 52/83A and most strains fell into group I and a mixed group of I &III. In addition, these types expressed were highly correlated with a resistance to ampicillin, chloramphenicol, streptomycin, nalidixic acid, kanamycin and methicillin. Plasmid DNA profiles from these isolates exhibited different patterns. The plasmid of mol wt. 27×106 was associated with resistant to ampicillin and kanamycin, whereas , a plasmid of mot wt. 30×106 effected resistance to ampicillin, kanamycin and streptomycin. The determinant which conferred resistance to chloramphenicol was carried by a plasmid of mol wt. 3×106 and tetracycline resistance was encoded on a plasmid of mol wt. 2.8×106 or 28×106 in different isolated strains. Analysis of plasmids by HindIII digestion showed that plasmids of different molecular weight but with similar phenotypic characteristics often share common fragments.

並列關鍵字

Staphyococcus aureus ； antibiotic resistance ； plasmid

國際替代計量

金黃色葡萄球菌之抗性及其質體之探討

全文下載

主題瀏覽