鱟變形細胞之培養

Cultivation of Horseshoe Crab Amebocytes

陳英俊(Ing-Jun Chen) ； 洪秀貞(Show-Jen Hong) ； 陳幼敏(Yow-Ming Chen) ； 楊玉嬌(Yu-Chiao Yang)

《The Kaohsiung Journal of Medical Sciences》 5卷9期 (1989/09) Pp. 516-521

https://doi.org/10.6452/KJMS.198909.0516

摘要

鱟的變形細胞所製成之鱟試劑為熱原試驗之方法，可廣泛地應用於生物檢體中內毒素之測定。為提高鱟試劑的品及產量，而嘗試以實驗室法來培養鱟變形細胞。由實驗結果顯現，鱟變形細胞之離體培養以15℃，pH=6.8~7.2為宜，且鱟的hemolymph是最佳培養基。如以放射標誌之thymidine測定DNA合成，得知變形細胞之DNA沒有複製現像。再嘗試鱟自體內之培養，發現經放血後而成之貧血鱟經三個月以上之養殖後變形細胞之個數已恢復至未失血前之變形細胞數，經鱟試劑之製備過程證明，可作為鱟試劑之來源。於鱟變形細胞之離體培養過程中發現有駐血原蟲存於鱟血中。有些離體的變形細胞可與駐血原蟲並存，有些個體則不然，駐血原蟲可能影響變形細胞的生存。以metronidazole及primaquine有抑制此原蟲之增殖作用，而quinine及pyrimethamine則無顯著效果。

並列摘要

The in vitro and in vivo cultivation of horseshoe crab amebocytes is studied. Partial bleeding promotes the proliferation of horseshoe crab amebocytes, and could be a useful method for amebocyte production in vivo. The optimal conditions of in vitro cultivation of amebocytes were incubation at 15℃ and pH△6.8-7.2. The best medium was the hemolymph of the horseshoe crab. Measuring DNA synthesis of amebocytes by H-thymidine uptake showed that no proliferation of amebocytes occurs during in vitro cultivation. Because some protozoa were found in the hemolymph, primaquine, quinine, pyrimethamine and metronidazole were used in the culture medium. Primaquine and metronidazole have a protozoatidal effect on the protozoa parasitising in the horseshoe crab.

並列關鍵字

horseshoe crab amebocytes ； cultivation ； protozocidal effect